Hpx 87h organic acids column

Manufactured by Bio-Rad

The HPX-87H organic acids column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of organic acids. It features a strongly acidic cation-exchange resin as the stationary phase, which allows for the separation of a wide range of organic acids, including acetic, formic, lactic, and citric acids.

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Lab products found in correlation

Differential refractive index detector, by Agilent Technologies (2 mentions) Agilent 1100 series hplc, by Agilent Technologies (1 mentions) Cation h guard cartridge, by Bio-Rad (1 mentions) Amicon ultrafree mc, by Merck Group (1 mentions)

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HPX-87H column (129 citations) HPX-87H (62 citations) 87H column (6 citations) HPX-87H organic acid column (2 citations)

4 protocols using hpx 87h organic acids column

HPLC Analysis of Fermentation Byproducts

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Concentration of furfural, glucose, xylose, acetate, and ethanol were determined by HPLC from 0.2 μm-filtered samples taken at different time points during fermentation using Agilent1100 series HPLC (Agilent, CA). BioRad HPX-87H organic acids column and Cation H⁺ guard cartridge (Bio-Rad, CA) were used in this study with an operation temperature of 55°C. A refractive index detector (RID) was used for compound detection. Dilute sulfuric acid (0.01 N) was used as the isocratic mobile phase at a flow rate of 0.6 mL/min, following published procedures (Chen et al., 2016 ).

Yang S., Franden M.A., Wang X., Chou Y.C., Hu Y., Brown S.D., Pienkos P.T, & Zhang M. (2020). Transcriptomic Profiles of Zymomonas mobilis 8b to Furfural Acute and Long-Term Stress in Both Glucose and Xylose Conditions. Frontiers in Microbiology, 11, 13.

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Quantitative Analysis of TCA Metabolites

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In addition to the NMR analysis to monitor glutarate, 2OG, 2HG, and succinate (Fig. 5) and the OPD assay to monitor 2OG (Fig. 6), we measured the consumption of 2OG or glutarate and production of succinate or 2HG by HPLC (HPX-87H organic acids column, Bio-Rad). Reaction mixtures (containing enzyme and reactants in the buffer specified) were incubated for various times at 30 °C, and quenched by adding 5 μl of 6 M sulfuric acid to the 300 μl aliquots. After centrifugation at 10,000 g for 5 min the supernatants were centrifuged in spin columns (Amicon ultrafree-MC from Millipore) at 10,000 g for 1 min, and 200 μl aliquots were injected onto the organic acids HPLC column that had been previously equilibrated with 13 mM sulfuric acid. The refractive index and absorbance at 214 nm were monitored and the integrated peak intensities were compared to those of authentic standards.

Herr C.Q., Macomber L., Kalliri E, & Hausinger R.P. (2019). Glutarate L-2-Hydroxylase (CsiD/GlaH) is an Archetype Fe(II)/2-Oxoglutarate-Dependent Dioxygenase. Advances in protein chemistry and structural biology, 117, 63-90.

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Measuring Fermentation Metabolites via HPLC

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Analysis of fermentation production was measured via high performance liquid chromatography (HPLC). We used a Bio-rad HPX-87H organic acids column with 5 mM H₂SO₄ as the eluent and a flowrate of 0.4 mL/min at 50 °C. Organic acids were detected at 210 nm. Cell densities of the cultures were determined by measuring optical density at 600 nm (GENESYS 20 Visible Spectrophotometer). Cell density samples were diluted as necessary, to fall within the linear range. A differential refractive index detector (Agilent, Santa Clara, CA) was used for analyte detection and quantification. Yields were calculated between two time points, whereas the cumulative yield was calculated between the initial and final measurements.

Pandit A.V., Harrison E, & Mahadevan R. (2021). Engineering Escherichia coli for the utilization of ethylene glycol. Microbial Cell Factories, 20, 22.

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Quantifying Fermentation Metabolites by HPLC

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Analysis of fermentation production was measured via high performance liquid chromatography (HPLC).
We used a Bio-rad HPX-87H organic acids column with 5 mM H 2 SO 4 as the eluent and a owrate of 0.4 mL/min at 50 °C. Organic acids were detected at 210 nm. Cell densities of the cultures were determined by measuring optical density at 600 nm (GENESYS 20 Visible Spectrophotometer). Cell density samples were diluted as necessary, to fall within the linear range. A differential refractive index detector (Agilent, Santa Clara, CA) was used for analyte detection and quanti cation. Yields were calculated between two time points, whereas the cumulative yield was calculated between the initial and nal measurements.

Pandit A.V., Harrison E., & Mahadevan R. (2020). Engineering Escherichia Coli for the Utilization of Ethylene Glycol.

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