MDA-MB-231 and BT549 cells were seeded at a density of 5 × 104 cells per chamber in an 8-µm-pore size transwell insert (Corning) coated with or without Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). Serum-free DMEM was added to the upper chamber, and DMEM containing 7% FBS was added to the lower chamber as the chemoattractant. Cells were incubated for 24 h, fixed in ice-cold methanol, and stained with a 1% crystal violet solution. Matrigel and any unmigrated cells were removed using cotton swabs prior to observation. For the wound-healing assay, cells were seeded in six-well plates and scratched with a 200 μL pipette tip. Culture media were refreshed to remove detached cells, and cell migration into the wound area was observed under an inverted microscope. Quantification of wound area was performed by using Image J software, version 1.51 (NIH, Betesda, MD, USA) [17 (link)].
8 m pore size transwell insert
Manufactured by Corning
Sourced in United States
The 8-µm-pore size transwell insert is a specialized laboratory equipment designed for cell culture applications. It features a porous membrane with 8-micrometer pores, which allows for the study of cell migration, invasion, and transport processes. The core function of this product is to facilitate the separation and monitoring of different cell populations within a shared culture environment.
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Lab products found in correlation
2 protocols using 8 m pore size transwell insert
1
Evaluating Cell Migration and Invasion in Breast Cancer
2
HepG2 Cell Migration Inhibition Assay
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HepG2 cells were evaluated in 8-µm pore size transwell insert (Corning). The bottom chambers of the transwell were filled with 600 µL of migration-inducing medium (DMEM medium with 10% FBS) and the top of each chamber was seeded with 2 × 10 4 HepG2 cells treated with different concentrations of TARAP (0, 0.25, 0.5 mg/mL). Cells were allowed to migrate for 24 hours at 37°C, and then removed from the upper compartment of the filter with a cotton swab. The filters were fixed with 4% paraformaldehyde for 30 minutes at room temperature, and then stained with 0.1% crystal violet. Percentage inhibition of migratory cells was quantified and expressed based on untreated control wells. 11, 12
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