Cxcl12dsred

Manufactured by Jackson ImmunoResearch

Cxcl12DsRed is a fluorescently labeled protein produced by Jackson ImmunoResearch. It is a fusion of the CXCL12 (also known as SDF-1) chemokine and the DsRed fluorescent protein, which emits red fluorescence. The core function of Cxcl12DsRed is to facilitate the visualization and tracking of CXCL12 in biological systems.

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5 protocols using cxcl12dsred

Detailed Mouse Experimental Protocols

Cited 5 times

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Experiments were conducted as sex unbiased, with randomly assigned 2-5 mice per experimental group. All mice were bred and maintained in a specific-pathogen-free barrier facility with free access to food and water. Both male and female mice were used between 16-36 weeks of age for most experiments, unless specified otherwise. All mouse studies and protocols were approved by the Institutional Animal Care and Use Committee (IACUC) at Albert Einstein College of Medicine. Wild-type C57BL6/J, Rosa26^{STOP-tdTomato} (AI14) (Madisen et al., 2010 (link)), Rosa26-CreERT2 (Ventura et al., 2007 (link)), CD4-Cre (Lee et al., 2001 (link)), Cxcl12^DsRed (Ding and Morrison, 2013 (link)), PA-GFP (Victora et al., 2010 (link)), APRIL^−/− (Xiao et al., 2008 (link)), Blimp1-YFP (Fooksman et al., 2010 (link)) and B1-8^hi (Shih et al., 2002 (link)) mice were used for most experiments and obtained from The Jackson Laboratory. Wild-type C57BL6 mice were used for PC-intrinsic-age-related CXCR4 level studies and obtained from the Charles River Laboratories.

Benet Z., Jing Z, & Fooksman D.R. (2021). Plasma cell dynamics in the bone marrow niche. Cell reports, 34(6), 108733.

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Genetically Modified Mouse Models for Vascular Research

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Animal experiments were approved by the Institutional Animal Care and Use Committee of Keio University (protocol no. A2021-003) and were performed in accordance with the Guidelines of Keio University for Animal and Recombinant DNA Experiments (protocol no. D2005-044). Cdh5-BAC-Cre^ERT2 (ref. ^{39 (link)}), Osx-Cre^ERT2 (ref. ^{8 (link)}), Vegfr2-flox^{40 (link)}, Dll4-flox^{41 (link)}, Col1a1-flox^{42 (link)}, CAG-LSL-EGFP^{43 (link)}, Vegfr1-BAC-DsRed^{44 (link)}, Vegfr2-BAC-GFP^{44 (link)} and Rbpj-flox^{45 (link)} mice had been developed previously. All mice were crossed with C57BL/6J mice more than 8× and maintained. For analyses of phenotypes, mice of both sexes were used. Csf1^op/op (stock no. 000231) and Cxcl12^+/DsRed (stock no. 0225458) mice were obtained from the Jackson Laboratory. For neonatal experiments of tamoxifen-inducible expression of Cre, 40 μg of 4-hydroxytamoxifen (4OHT, Sigma-Aldrich, catalogue no. H6278) was subcutaneously injected at the indicated timepoints. For adult experiments of tamoxifen-inducible expression of Cre, 1 mg of tamoxifen (Sigma-Aldrich, catalogue no. H5648) was subcutaneously injected at the indicated timepoints.

Iga T., Kobayashi H., Kusumoto D., Sanosaka T., Fujita N., Tai-Nagara I., Ando T., Takahashi T., Matsuo K., Hozumi K., Ito K., Ema M., Miyamoto T., Matsumoto M., Nakamura M., Okano H., Shibata S., Kohyama J., Kim K.K., Takubo K, & Kubota Y. (2023). Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis. Nature Cell Biology, 25(10), 1415-1425.

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Detailed Mouse Experimental Protocols

Cited 5 times

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Benet Z., Jing Z, & Fooksman D.R. (2021). Plasma cell dynamics in the bone marrow niche. Cell reports, 34(6), 108733.

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Standardized Mouse Husbandry and Genetic Strains

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All mouse husbandry and experiments were performed in accordance with Stanford Institutional Animal Care and Use Committee (IACUC) guidelines. All mice were housed in a pathogen-free facility. A maximum of 5 adult mice were housed per cage. Animals were monitored daily for normal husbandry and any health concerns. Cages were kept under a 12-hour light and 12-hour dark cycle. Mouse lines used in this study were: wild type (CD1, Charles River Laboratories, Strain Code #022), Cxcl12^fl/fl (The Jackson Laboratory, B6(FVB)-Cxcl12^tm11Link/J, Stock# 021773), Cxcr4^fl,fl (The Jackson Laboratory, B6.129P2-Cxcr4^tm27zo/J, Stock# 008767), Rosa26^tdTomato Cre reporter (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-TdTomato)Hze}/J, Stock# 007909), Rosa26^ntdTomato Cre reporter (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sortm75.1^{(CAG-tdTomato}*^)Hze/J, Stock# 025106), Cxcl12-DsRed (The Jackson Laboratory, Cxcl12^tm21Sjm/J, Stock# 022458), pTRE-H2BGFP (The Jackson Tg^{(tetO-mSTm2RJ/GFP)47Efu}/J, Stock# 005104), BmxCreERT2^{22 (link)} All mice in this study were maintained on a mixed background.

D’Amato G., Phansalkar R., Naftaly J.A., Fan X., Amir Z.A., Coronado P.E., Cowley D.O., Quinn K.E., Sharma B., Caron K.M., Vigilante A, & Red-Horse K. (2022). Endocardium-to-coronary artery differentiation during heart development and regeneration involves sequential roles of Bmp2 and Cxcl12/Cxcr4. Developmental cell, 57(22), 2517-2532.e6.

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Genetic Manipulation of Murine Vascular Biology

Cited 2 times

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All mouse colonies were housed and bred in the animal facility at Stanford University in accordance with institutional animal care and use committee (IACUC) guidance. All mouse lines were described earlier: Cx40CreER (Miquerol et al., 2015 (link)), ApjCreER (Chen et al., 2014 (link)), Tie2::eGFP::Claudin5 (Knowland et al., 2014 (link); Lutz et al., 2017 (link)). Cdh5CreER transgenic mice (Wang et al., 2010 (link)). Cxcl12^fl/fl (The Jackson Laboratory, B6(FVB)-Cxcl12^tm1.1Link/J, Stock# 021773), Cxcr4^fl/fl (The Jackson Laboratory, B6.129P2-Cxcr4^tm2Yzo/J, Stock# 008767), Rosa26^TdTomato Cre reporter (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-TdTomato)Hze}/J, Stock# 007909) Rosa26^Zsgreen (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm6(CAG-zsGreen1)Hze}/J, Stock# 007906), and Cxcl12-DsRed (The Jackson Laboratory, Cxcl12^tm2.1Sjm/J, Stock# 022458). For neonatal studies, all Tamoxifen injections to activate Cre activity (6mgs in corn oil) were given intraperitoneally to nursing mothers. Due to mouse genetic inheritance, analyses were compiled from age-matched males and females from multiple litters.

Das S., Goldstone A.B., Wang H., Farry J., D’Amato G., Paulsen M.J., Eskandari A., Hironaka C.E., Phansalkar R., Sharma B., Rhee S., Shamskhou E.A., Agalliu D., de Jesus Perez V., Woo Y.J, & Red-Horse K. (2019). A unique collateral artery development program promotes neonatal heart regeneration. Cell, 176(5), 1128-1142.e18.

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