Cryo-sections from frozen livers were generated using a 1720 Cryostat (Leica Microsystems, Wetzlar, Germany). Section were fixed in 4% buffered formalin for 10 min. Rinsed 3x in dH2O, before staining in 0.7% (w/v) Oil Red O (Sigma) in propylene glycol for 10 min, rinsed 3x dH2O, and stained with hematoxylin (Thermo Fisher Scientific. Waltham. MA. USA) for 2 min. Finally, sections were rinsed 3x dH2O and mounted with ImmuMount (Thermo Fisher Scientific). Images were captured using an Olympus BX51 light microscope at 40x magnification with an Olympus DP25 digital color camera (Olympus Corporation, Tokyo, Japan). Three images were captured from each animal by a blinded investigator.
1720 cryostat
Manufactured by Leica
Sourced in Germany
The Leica 1720 Cryostat is a precision instrument designed for the preparation of frozen tissue sections. It features a stable, low-temperature environment for cutting and mounting samples, enabling accurate and consistent sample preparation for various analytical techniques.
Automatically generated - may contain errors
3 protocols using 1720 cryostat
1
Liver Cryosection Staining for Lipid
2
Histological Staining of Frozen Liver
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Cryo-sections from frozen liver samples were generated using a 1720 Cryostat (Leica Microsystems, Wetzlar, Germany) from 3 to 4 mice per group. The cry-sections were fixed in 4% buffered formalin for 10 min, rinsed 3× in dH2O, and stained in 0.7% (w/v) Oil Red O (Sigma) in propylene glycol for 10 min, rinsed 3× dH2O, and stained with hematoxylin (Thermo Fisher Scientific, Waltham, MA, USA) for 2 min. Finally, sections were rinsed 3× dH2O and mounted with ImmuMount (Thermo Fisher Scientific). Images were captured using an Olympus BX51 light microscope at 40× magnification with an Olympus DP25 digital colour camera (Olympus Corporation, Tokyo, Japan). Three images per section were captured by a blinded investigator, and representative images were selected.
3
Histological Analysis of Liver Lipid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryo-sections from frozen livers were generated using a 1720 Cryostat (Leica Microsystems, Wetzlar, Germany) from 3 mice per group. Sections were fixed in 4 % buffered formalin for 10 min, rinsed 3× in dH2O, before staining in 0.7 % (w/v) Oil Red O (Sigma) in propylene glycol for 10 min, rinsed 3× dH2O, and stained with hematoxylin (Thermo Fisher Scientific, Waltham, MA, USA) for 2 min. Finally, sections were rinsed 3× dH2O and mounted with ImmuMount (Thermo Fisher Scientific). Images were captured using an Olympus BX51 light microscope at 40× magnification with an Olympus DP25 digital colour camera (Olympus Corporation, Tokyo, Japan). Three images were captured from each animal by a blinded investigator.
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