Total protein was extracted from 80 mg frozen liver sample as previously described51 (link). The protein concentration was determined according to the manufacturer’s instructions of Pierce BCA Protein Assay kit (Rockford, IL, USA). Forty or sixty micrograms of protein were used for electrophoresis on a 7.5% or 10% SDS-PAGE gel. Western blot analysis for methionine adenosyltransferase 2B (MAT2B) (15952–1-AP, Proteintech, USA, diluted 1:1000), DNA (cytosine-5-)-methyltransferase 1 (DNMT1) (sc-20701, Santa Cruz, UK, diluted 1:200), GR (sc-1004, Santa Cruz, UK, diluted 1:200), 11β-HSD type 1 (11β-HSD1) (ab109554, Abcam, UK, diluted 1:1000), Type 2 11β-HSD (11β-HSD2) (sc-20176, Santa Cruz, UK, diluted 1:200), PPARα (ab2779, Abcam, UK, diluted 1:1000), mitochondrial transcription factor A (TFAM) (BS7319, Bioworld Technology, USA, diluted 1:1000), COX4 (AP0707, Bioworld Technology, USA, diluted 1:1000) were carried out according to the recommended protocols provided by the manufacturers. Images were captured by VersaDoc 4000MP system (Bio-Rad, USA) and the band density was analyzed with Quantity One software (Bio-Rad, USA).
Ab2779
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab2779 is a primary antibody product manufactured by Abcam. It is designed for use in various laboratory applications, such as immunohistochemistry and Western blotting. The core function of this product is to detect and bind to a specific target protein or antigen.
Automatically generated - may contain errors
Lab products found in correlation
Ab109554, by Abcam (1 mentions)
Sc 20701, by Santa Cruz Biotechnology (1 mentions)
Versadoc 4000mp system, by Bio-Rad (1 mentions)
Sc 20176, by Santa Cruz Biotechnology (1 mentions)
Bs7319, by Bioworld Technology (1 mentions)
Ap0707, by Bioworld Technology (1 mentions)
Sc 1004, by Santa Cruz Biotechnology (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
Ab85104, by Abcam (1 mentions)
Bicinchoninic acid (bca), by Merck Group (1 mentions)
Ab97023, by Abcam (1 mentions)
Ab23673, by Abcam (1 mentions)
Ab97051, by Abcam (1 mentions)
Csb e08117r, by Cusabio (1 mentions)
First strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using ab2779
1
Liver Protein Extraction and Western Blot Analysis
2
Enzyme-linked Immunoassays for Gut Hormones
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Enzyme-linked immunosorbent assay (ELISA) kits for ghrelin (CSB-E13167r), CCK (CSB-E08114r), and GLP-1 (CSB-E08117r) were purchased from Cusabio, China. A glucose estimation kit was purchased from AutoSpan diagnostics Ltd, India. Anti-ghrelin (ab15861), anti-ghrelin receptor (ab85104), anti-CCKAR (ab75153), anti-PPARα (ab2779), anti-PPARδ (ab23673) primary antibodies, and anti-rabbit/mouse horseradish peroxidase (HRP) conjugated secondary antibodies (ab97051, ab97023) were purchased from Abcam, Inc. First strand cDNA synthesis kit and other PCR reagents were purchased from Fermentas, USA. Primers were synthesised by Eurofins Genomics, India. β-actin primary antibody (A1978), Trizol, Bicinchoninic acid, and all other chemicals were purchased from Sigma Aldrich Co, LLC.
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