Lc prominence lc20ad

Manufactured by Shimadzu

The Shimadzu Prominence LC20AD is a high-performance liquid chromatography (HPLC) system. It is designed to provide reliable and accurate analysis of a wide range of samples. The core function of the LC20AD is to separate, identify, and quantify the components within a liquid sample.

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Lab products found in correlation

Ms ms api 5500, by AB Sciex (2 mentions) Cto 20a, by Shimadzu (2 mentions) Sil 20ac ht, by Shimadzu (2 mentions)

2 protocols using lc prominence lc20ad

HPLC-MS/MS Quantification of Analytes

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The HPLC-MSMS system was composed of an LC-Shimadzu prominence LC20AD (Shimadzu Scientific Instruments, Columbia, MD) with an ultramino 3 μm 150 × 2.1 mm 100A aminopropylsilane Restek column (Restek, Bellefonte, PA) with a flow rate of 0.30 mL/min with degasser and autosampler SIL-20AC HT (Shimadzu Scientific Instruments) and column oven CTO-20A (Shimadzu Scientific Instruments) run at 40°C. Mobile phase A was HPLC-grade water with 0.1% formic acid, and phase B was acetonitrile with 0.1% formic acid. Elution was programmed to start at 70% phase B for 0.5 minute, then fall to 5% B at 2 minutes, return to 70%B at 2.5 minutes, and equilibrate for 2 minutes before the next sample injection.
MS/MS-API 5500 (AB Sciex, Framingham, MA) with turbo-ion probe (ESI) operated at 600°C. The transitions monitored are listed with the retention times in Appendix Table 2A.

Lee G.O., Kosek P., Lima A.A., Singh R., Yori P.P., Olortegui M.P., Lamsam J.L., Oliveira D.B., Guerrant R.L, & Kosek M. (2014). Lactulose:Mannitol Diagnostic Test by HPLC and LC-MSMS Platforms: Considerations for Field Studies of Intestinal Barrier Function and Environmental Enteropathy. Journal of Pediatric Gastroenterology and Nutrition, 59(4), 544-550.

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Targeted Quantification of Analytes by HPLC-MS/MS

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HPLC-MSMS system was composed of a LC- Shimadzu prominence LC20AD (Shimadzu, Maryland), with an ultramino 3 um 150 x 2.1 mm 100A aminopropylsilaneRestek column (Restek, Bellafonte, PA) with a flow rate of 0.30 ml/min with degasser and auto-sampler SIL-20AC HT (Shimadzu, Maryland), and column oven CTO-20A (Shimadzu, MD) run at 40°C. Mobile phase A was HPLC grade water with 0.1% formic acid and phase B was acetonitrile with 0.1% formic acid. Elution was programmed to start at 70% phase B for 0.5 minutes, then fall to 5% B at 2 minutes, return to 70%B at 2.5 minutes and equilibrate for two minutes prior to the next sample injection. MS/MS-API 5500 (AB Sciex, US) with turbo-ion probe (ESI) operated at 600C. The transitions monitored described before are listed with the retention times in Table 1 [17 (link)].

Musa M.A., Kabir M., Hossain M.I., Ahmed E., Siddique A., Rashid H., Mahfuz M., Mondal D., Ahmed T., Petri W.A, & Haque R. (2019). Measurement of intestinal permeability using lactulose and mannitol with conventional five hours and shortened two hours urine collection by two different methods: HPAE-PAD and LC-MSMS. PLoS ONE, 14(8), e0220397.

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