Agilent 7820 gc

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 7820 GC is a gas chromatography system designed for reliable and efficient separation and analysis of a wide range of organic compounds. It features precise temperature and flow control, high-performance detectors, and intuitive software for data processing and analysis.

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Agilent 7820 GC with 5975 MSD Agilent 7820/5975 GC–MS system Agilent 7820

2 protocols using agilent 7820 gc

Photocatalytic CO2 Reduction Protocol

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Before photocatalysis,
a piece of 3DG–TiO₂ (dimension 2 × 2 ×
0.1 cm³) or 1 mg of P25 TiO₂ was placed in a
sealed test tube. The test tube was purged with a mixture of high-purity
CO₂ gas (99.999%, Airgas) and triethylamine vapor for 20
min. The sample was then irradiated with a 200 W mercury lamp. The
light intensity on the sample was fixed at 140 mW/cm².
The head space was sampled with a gas-tight syringe at different time
intervals for product analysis using Agilent 7820 GC, equipped with
a thermal conductivity detector and a 60/80 Carboxen-1000 packed column
(Supelco).

Park J., Jin T., Liu C., Li G, & Yan M. (2016). Three-Dimensional Graphene–TiO2 Nanocomposite Photocatalyst Synthesized by Covalent Attachment. ACS Omega, 1(3), 351-356.

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Quantification of Carbon Monoxide Release

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GC was performed on an Agilent 7820 GC (Agilent Technologies) equipped with a thermal conductivity detector (TCD) and a packed molecular sieve column Carboxen 1000, 15 ft × 2.1 mm (RESTEK, Bellefonte, PA, USA); helium was used as the carrier gas. Oven temperature was programed as follows: 35 °C in 5 min, then to 225 °C at a rate of 20 °C/min, and then hold for 5.5 min. TCD detector was set at 125 °C. Calibration curve was generated by injecting a series of varying volumes of pure CO gas taken from a gas sampling bag into a 6-mL head-space vial pre-sealed with 2.8 mL phosphate buffered saline (PBS)/DMSO (1:6, v:v) medium, which was the same volume used in the CO release experiment from the prodrug. After incubation at 37 °C overnight, 250 μL of the head-space gas was injected to the GC. The peak area of CO was normalized with the peak area of oxygen as the internal standard (from the air in the head space). The ratio between CO and O₂ was plotted against the injected pure CO molar quantity to give the calibration curve.

Bakalarz D., Surmiak M., Yang X., Wójcik D., Korbut E., Śliwowski Z., Ginter G., Buszewicz G., Brzozowski T., Cieszkowski J., Głowacka U., Magierowska K., Pan Z., Wang B, & Magierowski M. (2020). Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage. Acta Pharmaceutica Sinica. B, 11(2), 456-475.

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