Anti 4 1bb pe

Manufactured by BioLegend

Sourced in United States

Anti-4-1BB-PE is a monoclonal antibody that binds to the 4-1BB protein, also known as CD137, which is expressed on the surface of activated T cells, natural killer cells, and other immune cells. This antibody is conjugated to the fluorescent dye phycoerythrin (PE), which allows for the detection and analysis of 4-1BB-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Anti cd28 pe, by BD (1 mentions) Thermo cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Anti ctla4 pe, by BioLegend (1 mentions) Anti pd 1 pe, by BioLegend (1 mentions) Anti cd25 bv421, by BioLegend (1 mentions) Anti cd8 fitc, by BioLegend (1 mentions) Anti cd4 bv605, by BioLegend (1 mentions)

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4-1BB-PE (2 citations)

2 protocols using anti 4 1bb pe

Evaluating In Vitro Binding Capacity of Immune Checkpoint Proteins

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Stable cell lines were generated to evaluate in vitro binding capacity of anti-PD-1, CD80-Fc, and 4-1BBL-Fc proteins. For this purpose, total RNA was extracted from anti-CD3/CD28 activated human PBMCs, and the first-strand cDNA was synthesized by Thermo cDNA synthesis kit (Thermo Scientific, USA). Human PD-1 (hPD-1), CTLA-4 (hCTLA-4), CD28 (hCD28), and 4-1BB (h4-1BB) coding genes were amplified by PCR using specific primers and separately inserted into pCHO1.0 expression vector. As described earlier, these constructs were transfected to CHO-K1 by electroporation, and then stable cell lines were produced by puromycin/MTX strategy. The expression of these proteins on the surface of transfected CHO-K1 cells was screened by flow cytometry using anti-PD-1-PE (BioLegend™, USA), anti-CD28-PE (BD Pharmingen™, USA), anti-CTLA4-PE (BioLegend), and anti-4-1BB-PE (BioLegend™, USA) antibodies.

Kaviani E., Hosseini A., Mahmoudi Maymand E., Ramzi M., Ghaderi A, & Ramezani A. (2022). Triggering of lymphocytes by CD28, 4-1BB, and PD-1 checkpoints to enhance the immune response capacities. PLOS ONE, 17(12), e0275777.

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Multi-Marker Immune Cell Profiling

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Five × 10⁵ unstimulated and PHA (4 μg/mL)
stimulated PBMCs were stained on ice for 30 min with anti-4-1BB PE, anti-4-1BBL
APC, anti-CD4 BV605, anti-CD8 FITC and anti-CD25 BV421, all from BioLegend. For
4-1BBL binding, PBMCs were incubated first with chimeric CD137L:muCD8 fusion
protein (Axxora ANC-503) then washed and incubated with murine anti-CD8 PE.

Alosaimi M., Hoenig M., Jaber F., Platt C.D., Jones J., Wallace J., Debatin K.M., Schulz A., Jacobsen E., Möller P., Shamseldin H.E., Abdulwahab F., Ibrahim N., Alardati H., Almuhizi F., Abosoudah I.F., Basha T.A., Chou J., Alkuraya F.S, & Geha R.S. (2019). Immunodeficiency and Epstein Barr virus induced lymphoproliferation caused by 4-1BB deficiency. The Journal of allergy and clinical immunology, 144(2), 574-583.e5.

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