Mouse monoclonal anti nos3 a 9

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Mouse monoclonal anti-NOS3 (A-9) is an antibody produced by Santa Cruz Biotechnology. It is designed to detect NOS3 (nitric oxide synthase 3) expression in various samples.

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Lab products found in correlation

Amersham imager 600, by GE Healthcare (1 mentions) Ecl prime western blotting system, by Cytiva (1 mentions) Mouse monoclonal anti β actin, by Santa Cruz Biotechnology (1 mentions) Rabbit monoclonal anti nox4, by Abcam (1 mentions) Rabbit monoclonal anti nox2, by Abcam (1 mentions) Amersham imager, by Cytiva (1 mentions)

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Anti-NOS3 (3 citations) Monoclonal mouse anti (2 citations)

2 protocols using mouse monoclonal anti nos3 a 9

Immunohistochemical Analysis of NOS2 and NOS3

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Immunohistochemistry was performed following standard pathology protocols in deparaffinized BM tissues from deidentified patients using mouse monoclonal anti-NOS2 (C-11) and mouse monoclonal anti-NOS3 (A-9) both from Santa Cruz Biotechnology. Evaluation and scoring were independently made by at least two investigators. Percentage of positive tumor cells and intensity of staining (by using an ad hoc scale from 1 to 4) were used to determine the total score.

Marullo R., Castro M., Yomtoubian S., Calvo-Vidal M.N., Revuelta M.V., Krumsiek J., Cho A., Morgado P.C., Yang S., Medina V., Roth B.M., Bonomi M., Keshari K.R., Mittal V., Navigante A, & Cerchietti L. (2021). The metabolic adaptation evoked by arginine enhances the effect of radiation in brain metastases. Science Advances, 7(45), eabg1964.

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Western Blot Characterization of Oxidative Stress Markers

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Following SDS-PAGE, proteins were transferred to nitrocellulose membrane and incubated with specific antibodies: mouse monoclonal anti-TGF-β1 (3C11) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:3000 dilution); rabbit polyclonal anti-MOX1 (H-75) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:1000 dilution); rabbit monoclonal anti-NOX2 (Epitomics-Abcam, Burlingame, CA, USA; 1:8000 dilution); rabbit monoclonal anti-NOX4 (Epitomics-Abcam, Burlingame, CA, USA; 1:7500 dilution); mouse monoclonal anti-NOS3 (A-9) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:2000 dilution); purified mouse anti-eNOS (pS1177) (BD Transduction Laboratories, 1:2000 dilution); mouse monoclonal anti-p-NOS3 (pt495.33) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:2000 dilution); mouse monoclonal anti-3-nitrotyrosine (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:1000 dilution). Mouse monoclonal anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:20,000 dilution) was also used for protein loading control. Adequate anti-rabbit or anti-mouse secondary antibodies were used following the manufacturer’s recommendations, and signals were revealed with ECL™ Prime Western Blotting System (Amersham, RPN2232) and measured with an Amersham Imager 600 (GE Healthcare Life Science).

Reyes-Goya C., Santana-Garrido Á., Soto-Astacio E., Aramburu Ó., Zambrano S., Mate A, & Vázquez C.M. (2020). Mechanism of Vascular Toxicity in Rats Subjected to Treatment with a Tyrosine Kinase Inhibitor. Toxics, 8(3), 49.

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