Normal adult human epithelial keratinocytes heka

Manufactured by ScienCell

Normal adult human epithelial keratinocytes (HEKa) are primary cells isolated from healthy adult human skin. They are a key component of the epidermis and play a critical role in skin structure and function.

Automatically generated - may contain errors

Lab products found in correlation

Glass coverslip, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum 4 6 diamidino 2 phenylindole dapi, by Merck Group (2 mentions) Nunclon δ surface multidishes, by Thermo Fisher Scientific (2 mentions) Tween 20, by Merck Group (2 mentions) Sodium bicarbonate, by Merck Group (2 mentions) Triton x 100, by Merck Group (2 mentions) Sodium azide, by Merck Group (2 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (2 mentions) Goat serum, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Menadione, by Merck Group (1 mentions) Trypan blue, by Merck Group (1 mentions) Dispase 2, by Merck Group (1 mentions) Soybean trypsin inhibitor, by Merck Group (1 mentions) Gentamicin, by Merck Group (1 mentions) Cnt prime, by CELLnTEC (1 mentions) Gentamycin, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Keratinocyte medium, by ScienCell (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)

2 protocols using normal adult human epithelial keratinocytes heka

Cultivation of Normal Human Epidermal Keratinocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Normal adult human epithelial keratinocytes (HEKa) were obtained from ScienCell Research Laboratories (San Diego, CA). The defined proprietary culture medium, CNT-Prime, was obtained from Cellntec Advanced Cell Systems AG (Bern). Goat serum, trypsin-ethylenediaminetetraacetic acid (EDTA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium bicarbonate, sodium azide, Tween-20, Triton X-100, gentamicin, bovine serum albumin (BSA), fetal bovine serum (FBS), 4′,6-diamidino-2-phenylindole (DAPI), dispase II, soybean trypsin inhibitor, DHR, DHE, menadione and trypan blue were purchased from Sigma Aldrich (St Louis, MO). Nunclon Δ surface multidishes, glass coverslips, pipettes and other routine plastics were obtained from Thermo Fisher Scientific (Waltham, MA). Phosphate buffered saline (PBS), Hanks balanced salt solution (HBSS) and minimum essential medium (MEM) were from Life Technologies (Carlsbad, CA).

Jackson C.J., Reppe S., Eidet J.R., Eide L., Tønseth K.A., Bergersen L.H., Dartt D.A., Griffith M, & Utheim T.P. (2017). Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets. Scientific Reports, 7, 8206.

+ Open protocol

+ Expand

Cultivating Human Epithelial Keratinocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Normal adult human epithelial keratinocytes (HEKa) and Keratinocyte Medium (KM) were obtained from ScienCell Research Laboratories (San Diego, CA). The provided cells originated from an 18 year old female undergoing breast-reduction surgery. Goat serum, trypsin-ethylenediaminetetraacetic acid (EDTA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium bicarbonate, sodium azide, Tween-20, Triton X-100, gentamycin, bovine serum albumin (BSA), fetal bovine serum (FBS), 4', 6-diamidino-2-phenylindole (DAPI), were purchased from Sigma Aldrich (St Louis, MO). Nunclon Δ surface multidishes, glass coverslips, pipettes and other routine plastics were obtained from Thermo Fisher Scientific (Waltham, MA). Phosphate buffered saline (PBS) and minimum essential medium (MEM) were from Life Technologies (Carlsbad, CA).

Jackson C., Aabel P., Eidet J.R., Messelt E.B., Lyberg T., von Unge M, & Utheim T.P. (2014). Effect of Storage Temperature on Cultured Epidermal Cell Sheets Stored in Xenobiotic-Free Medium. PLoS ONE, 9(8), e105808.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!