Hoechst solution 33 258

Recombinant Human ACE2 Protein (933-ZN) was purchased from R&D Systems (Minneapolis, MN, USA) and stored in a buffer according to the manufacturer’s instructions. Monoclonal mouse anti-human ACE2 Antibody (MAB933) was purchased from R&D Systems (Minneapolis, MN, USA). Human recombinant VE-cadherin extracellular domain (EC1-5) was produced in the laboratory. Peroxidase AffiniPure Goat Anti-Mouse IgG (H + L) (AB-2338447), Peroxidase AffiniPure Goat Anti-Rabbit IgG (H + L) (AB-2307391), and Cy3™ AffiniPure Goat Anti-Mouse IgG (H + L) (AB-2338680) were all purchased from Jackson Immunoresearch (Ely, Cambridgeshire, UK). Hoechst solution (33,258) was purchased from Sigma Aldrich. The mouse monoclonal anti-human VE-cadherin antibody (clone BV9) and the rabbit polyclonal anti-EC1 anti-human VE-cadherin antibody were produced in the laboratory.

HaloTag R110Direct Ligand G3221 and HaloTag TMRDirect Ligand G2991 were purchased from Promega. Hoechst solution (33,258; used at 1:1000 dilution), L-histidinol dihydrochloride (H6647; used at 2 mM), all-trans-Retinoic acid (R-2625; used at 10 μm), thapsigargin (T-9033; used at 300 nM), and triptolide (T-3652, used at 500 μM) were all from Sigma. α-Amanitin (used at 10 μM) was purchased from Sigma (A2263) or EMD/Millipore (129741). FuGENE HD transfection reagent was obtained from Promega.