Stattic

Manufactured by R&D Systems

Sourced in United States

Stattic is a small molecule inhibitor of STAT3 phosphorylation. It blocks the activation of STAT3, a transcription factor involved in various cellular processes.

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Lab products found in correlation

Niclosamide, by Merck Group (1 mentions) Adam12, by Proteintech (1 mentions) Gapdh, by Merck Group (1 mentions) Ag1478, by Merck Group (1 mentions) Pd98059, by Santa Cruz Biotechnology (1 mentions) Ly294002, by R&D Systems (1 mentions) Il 6r, by R&D Systems (1 mentions) Lipopolysaccharides (lps), by R&D Systems (1 mentions) Tnf α, by R&D Systems (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Su16f, by R&D Systems (1 mentions) 2 mercaptoethanol, by Bio-Rad (1 mentions) Ammonium persulfate, by Bio-Rad (1 mentions) Recombinant human jagged 1, by R&D Systems (1 mentions) Tween 20, by Bio-Rad (1 mentions) 2 1h indol 3 ylcarbonyl 4 thiazolecarboxylic acid methyl ester ite, by Bio-Techne (1 mentions) Polyvinylidene difluoride pvdf, by Bio-Rad (1 mentions) Dmem f12, by American Type Culture Collection (1 mentions)

4 protocols using stattic

Signaling Pathway Regulation in Angiogenesis

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Antibodies used include: ADAM12 (Proteintech Group, Chicago, IL), CD31 (BD Biosciences, San Jose, CA) thrombospondin-1 (Neomarkers, Freemont, CA), phosphoSTAT3/total STAT3, phosphoMAPK/total MAPK, phosphoAkt/totalAkt and phosphoVEGR2/total VEGFR2 (Cell Signaling, Danvers, MA), GAPDH (Millipore, Temecula, CA), HRP-conjugated anti-rabbit and anti-mouse antibodies (Vector Biolabs, Burlingame, CA). Small molecule inhibitors include: EGFR inhibitors AG1478 (CAS #175178-82-2, EMD Millipore, Billerica, MA) and PD15035 (CAS# 183322-45-4, R & D Systems, Minneapolis, MN) STAT3 inhibitors; Stattic (CAS# 19983-44-9, R & D Systems) and Niclosamide (CAS# 50-65-7, Sigma Aldrich, St. Louis, MO). VEGF antibody (Ranibizumab, Genetech, San Francisco, CA).

Roy R., Dagher A., Butterfield C, & Moses M.A. (2017). ADAM12 is a Novel Regulator of Tumor Angiogenesis via STAT3 Signaling. Molecular cancer research : MCR, 15(11), 1608-1622.

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Inflammatory Signaling in Endothelial Cells

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HUVECs (3 × 10⁵ cells/well for 6-well plates and 6 × 10⁴ cells/well for 24-well plates) were plated overnight in complete endothelial medium containing antibiotics. The next day, the medium was replaced with fresh antibiotics free medium and cells were treated with different concentrations of IL-6, IL-6R, TNF-α and LPS (all from R&D systems, USA) and/or pharmacological inhibitors CP690550, Stattic and LY294002 (all from R&D systems, USA), and PD98059 (Santa Cruz biotechnology, USA) for different time points ranging from 5 min to 48 h. At the end of incubations, supernatants and cells were collected and kept at -80 °C until further analysis. The cells were used to extract total protein or RNA.

Zegeye M.M., Lindkvist M., Fälker K., Kumawat A.K., Paramel G., Grenegård M., Sirsjö A, & Ljungberg L.U. (2018). Activation of the JAK/STAT3 and PI3K/AKT pathways are crucial for IL-6 trans-signaling-mediated pro-inflammatory response in human vascular endothelial cells. Cell Communication and Signaling : CCS, 16, 55.

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Isolation and Treatment of Brain Cells

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Human brain microvascular pericytes (ScienCell Research Laboratories [Carlsbad, CA]), BMDMs, and oligodendrocyte precursor cells (OPCs) were used as described previously 2, (link)22 (link) and in Methods in the Data Supplement. SU16f (10 nmol/L, R&D Systems #3304, Minneapolis, MN) and stattic (1 μmol/L, R&D Systems #19983-44-9) were used as a selective PDGFRβ inhibitor and STAT3 (signal transducer and activator of transcription 3) inhibitor. MD 19 (link) and conditioned medium 2 were prepared as described in Methods in the Data Supplement.

Shibahara T., Ago T., Tachibana M., Nakamura K., Yamanaka K., Kuroda J., Wakisaka Y, & Kitazono T. (2020). Reciprocal Interaction Between Pericytes and Macrophage in Poststroke Tissue Repair and Functional Recovery. Stroke, 51(10).

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TCDD and ITE Signaling Pathway

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2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) in dimethyl sulfoxide (DMSO) was purchased from Cambridge Isotopes Laboratory (Andover, MA). 2-(1H-Indol-3-ylcarbonyl)-4-thiazolecarboxylic acid methyl ester (ITE) was purchased from Tocris Bioscience (Bristol, United Kingdom). STATTIC and recombinant human JAGGED1 were purchased from R&D Systems (Minneapolis, MN). Non-targeting short interfering RNA (siRNA) (D-001810-01-20), ON-TARGETplus siRNA against AHR (J-004990-08-0010) and JAG1 (J-011060-11-0005) were purchased from GE Dharmacon (Lafayette, CO). The following supplies were purchased from ThermoFisher Scientific (Pittsburgh, PA): Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12), fetal bovine serum (FBS), phosphate buffered saline (PBS), penicillin/streptomycin (P/S), dimethyl sulfoxide (DMSO), TrypLE Express and Lipofectamine RNAiMAX. Sodium dodecyl sulfate (SDS), 30% acrylamide/bis solution, ammonium persulfate, Tween-20, 2-mercaptoethanol and polyvinylidene difluoride (PVDF) membranes were obtained from BIO-RAD (Hercules, CA). Cell lines were purchased from the American Type Culture Collection (Manassas, VA) and cultured in DMEM:F-12 supplemented with 10% FBS and P/S at 37 °C with 5% CO₂.

Piwarski S.A., Thompson C., Chaudhry A.R., Denvir J., Primerano D.A., Fan J, & Salisbury T.B. (2020). The putative endogenous AHR ligand ITE reduces JAG1 and associated NOTCH1 signaling in triple negative breast cancer cells. Biochemical pharmacology, 174, 113845.

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