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Ammonium bicarbonate abc

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Ammonium bicarbonate (ABC) is a chemical compound with the formula (NH4)HCO3. It is a white crystalline solid that is commonly used as a food additive and in some laboratory applications. ABC's core function is to act as a pH buffer, regulating the acidity or basicity of a solution.

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3 protocols using ammonium bicarbonate abc

1

Glycoproteomic analysis of PANC-1 cells

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Dithiothreitol (DTT), PNGase F and sequencing grade trypsin were from Promega (Madison, WI). Optimal LC/MS grade acetonitrile (ACN), methanol (MeOH) and water were from Fisher Scientific (Pittsburgh, PA). Concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinuscommunis agglutinin (RCA120), iodoacetamide (IAA), acetyl-D18 glucosamine, D-lactose, methyl α-D-mannopyranoside and manganese dichloride were obtained from Sigma-Aldrich (St. Louis, MO). Tris base, urea (UA), sodium chloride, ammonium bicarbonate (ABC) and calcium chloride (CaCl2) were obtained from Fisher Scientific (Pittsburgh, PA). Trifluoroacetic acid (TFA), triethylammonium bicarbonate (TEAB), N, N-dimethylformamide (DMF), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium tetrafluoroborate (DMTMM), N-methylmorpholine (NMM) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Hydroxylamine solution was purchased from Alfa Aesar (Ward Hill, MA). C18 OMIX tips were obtained from Agilent (Santa Clara, CA). Hydrophilic interaction chromatography material (PolyHYDROXYETHYL A) was obtained from PolyLC (Columbia, MD). Microcon filters YM-30 (30 kDa) was purchased from Merck Millipore (Billerica, MA). PANC-1 pancreatic ductal adenocarcinoma cells were from ATCC (Manassas, VA). Duplex DiLeu tags were custom synthesized in our own lab.45 (link)
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2

Quantitative Proteomics of ApoA-1

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All reagents and solvents were LC–MS grade quality unless otherwise noted. The gene for Human ApoA-1 in the Gateway entry vector pFN21AE0099, FuGENE® 6 transfection reagent, protease inhibitors, and mammalian cell lysing buffer purchased from Promega (Madison, WI, USA). Human ApoA-1 isolated from human HDL was from ProSpec (East Brunswick, NJ, USA). Mouse serum was from Sigma-Aldrich (St. Louis, MO, USA). SILAC amino acids including [13C615N2]-lysine and [13C915N1]-tyrosine were from Cambridge Isotope Laboratories (Andover, MA, USA). DL-dithiothreitol (DTT), iodoacetamide (IAA) and dialyzed fetal bovine serum was from Sigma-Aldrich. 2-mecaptoethanol (BME) was from BioRad (Hercules, CA, USA). Ammonium bicarbonate (ABC) and formic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA). Colloidal Coomassie and sequencing-grade modified trypsin from Promega. LC grade water and acetonitrile were from Burdick and Jackson (Muskegon, MI, USA). Custom-made AQUA peptides corresponding to endogenous peptides of trypsin-digested ApoA-1 were purchased from Thermo Scientific (Isotopic enrichment >99%, HeavyPeptide AQUA custom synthesis service (Rockford, IL, USA).
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3

Quantitative Proteomics of ApoA-1

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All reagents and solvents were LC–MS grade quality unless otherwise noted. The gene for Human ApoA-1 in the Gateway entry vector pFN21AE0099, FuGENE® 6 transfection reagent, protease inhibitors, and mammalian cell lysing buffer purchased from Promega (Madison, WI, USA). Human ApoA-1 isolated from human HDL was from ProSpec (East Brunswick, NJ, USA). Mouse serum was from Sigma-Aldrich (St. Louis, MO, USA). SILAC amino acids including [13C615N2]-lysine and [13C915N1]-tyrosine were from Cambridge Isotope Laboratories (Andover, MA, USA). DL-dithiothreitol (DTT), iodoacetamide (IAA) and dialyzed fetal bovine serum was from Sigma-Aldrich. 2-mecaptoethanol (BME) was from BioRad (Hercules, CA, USA). Ammonium bicarbonate (ABC) and formic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA). Colloidal Coomassie and sequencing-grade modified trypsin from Promega. LC grade water and acetonitrile were from Burdick and Jackson (Muskegon, MI, USA). Custom-made AQUA peptides corresponding to endogenous peptides of trypsin-digested ApoA-1 were purchased from Thermo Scientific (Isotopic enrichment >99%, HeavyPeptide AQUA custom synthesis service (Rockford, IL, USA).
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