Cellular protein extracts were prepared as previously described [7 (link)]. Equal protein samples (40μg) were separated on 10% (15% for analysis of RhoC or 7.5% for analysis of NF1) SDS polyacrylamide gels and analyzed by Western blotting using Odyssey Blocking Buffer (LI-COR Biosciences) and the following antibodies: anti c-Jun and anti E-cadherin (Transduction Laboratories), anti RhoC (Abcam), anti ERK and anti phospho ERK (Sigma), anti-JNK, anti p38, anti phospho-c-Jun and anti c-Fos (Santa Cruz Biotechnology), anti phospho-JNK and anti phospho-p38 (Cell Signaling) anti GFP (Covance) and anti neurofibromin (Bethyl Laboratories). Anti-mouse or anti-rabbit IgG coupled to IRDye 800CW (LI-COR Biosciences) was used as secondary antibody, and protein bands were visualized by the Odyssey infrared imaging system (LI-COR Biosciences). Bend intensity was determined using the Odyssey software (LI-COR Biosciences).
Anti neurofibromin
Manufactured by Fortis Life Sciences
Sourced in United States
Anti neurofibromin is a laboratory reagent used for the detection and quantification of neurofibromin, a protein involved in the regulation of cell growth and division. It is commonly used in research related to neurofibromatosis, a genetic disorder affecting the nervous system.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey software, by LI COR (1 mentions)
Anti jnk, by Merck Group (1 mentions)
Anti c fos, by Santa Cruz Biotechnology (1 mentions)
Irdye 800cw, by LI COR (1 mentions)
Anti phospho c jun, by Santa Cruz Biotechnology (1 mentions)
Odyssey blocking buffer, by LI COR (1 mentions)
Anti p38, by Santa Cruz Biotechnology (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Anti gfp, by Fortrea (1 mentions)
Wortmannin, by Cayman Chemical (1 mentions)
Anti gapdh, by Novus Biologicals (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti phospho akt xp, by Cell Signaling Technology (1 mentions)
2 protocols using anti neurofibromin
1
Western Blot Analysis of Cellular Proteins
2
Antibody-Based Endothelial Signaling Analysis
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The following antibodies were used: anti-neurofibromin (#A300; Bethyl Laboratories, Montgomery, TX, USA); anti-phospho-Akt XP (#4060), anti-Akt (#2938S), anti-phospho-Erk XP (#4370), anti-Erk (#4695S; Cell Signaling, Danvers, MA, USA); anti-phospho-endothelial nitric oxide synthase (eNOS) (612392) and FITC-anti-CD31 (BD Laboratories, San Jose, CA, USA); and anti-GAPDH (Novus, Littleton, CO, USA). Recombinant human and murine VEGF were purchased from Peprotech (Rocky Hill, NJ, USA). Wortmannin was purchased from Cayman Chemicals (Ann Arbor, MI, USA).
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