Total cholesterol, alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin and direct bilirubin, total protein, albumin, urea, creatinine, calcium, sodium, chloride, potassium, and bicarbonate test kits were purchased from Randox Laboratory (United Kingdom).
Alkaline phosphatase
Manufactured by Randox
Sourced in United Kingdom
Alkaline phosphatase is a laboratory assay used to measure the activity of the enzyme alkaline phosphatase in biological samples. Alkaline phosphatase is an enzyme involved in the breakdown of various molecules and is found in various tissues throughout the body.
Automatically generated - may contain errors
Lab products found in correlation
Total cholesterol, by Randox (1 mentions)
Total bilirubin, by Randox (1 mentions)
Total protein, by Randox (1 mentions)
Direct bilirubin, by Randox (1 mentions)
Alanine aminotransferase, by Randox (1 mentions)
Gamma glutamyl transferase, by Randox (1 mentions)
Aspartate transaminase, by Randox (1 mentions)
Alanine transaminase, by Randox (1 mentions)
Urea, by Randox (1 mentions)
Creatinine, by Randox (1 mentions)
4 protocols using alkaline phosphatase
1
Comprehensive Biochemical Profiling Protocol
2
Comprehensive Serum Biomarker Analysis
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Serum alanine aminotransferase (ALT) (Randox, UK), alkaline phosphatase (AP) (Randox, USA) and aspartate aminotransferase (AST) (Amsbio, Switzerland) activities were determined by colorimetry as described in the laboratory manual of Collaborative Studies Clinical Laboratory, Minnesota (Eckfeldt 2007).
Serum creatinine levels were estimated according to those of (Toora and Rajagopal 2002), blood urea modified after the methods of Fawcett and Scott (Fawcett and Scott 1960) simply by using serum instead of plasma, Serum total cholesterol (TC) was determined by the methods of Allain et al.(Allain et al. 1974) and high density lipoproteins (HDL-C) were measured by Randox diagnostic test kit a method for direct measurement of HDL-C (Randox, UK), while serum triglyceride (TG) was determined by the methods of (Fossati and Prencipe 1982). Low density lipoproteins (LDL-C) concentration in the samples was determined by the methods of (Hoffmann et al. 1985), which was not significantly variant to those calculated with Friedwald’s equation (Friedewald et al. 1972).
Serum creatinine levels were estimated according to those of (Toora and Rajagopal 2002), blood urea modified after the methods of Fawcett and Scott (Fawcett and Scott 1960) simply by using serum instead of plasma, Serum total cholesterol (TC) was determined by the methods of Allain et al.(Allain et al. 1974) and high density lipoproteins (HDL-C) were measured by Randox diagnostic test kit a method for direct measurement of HDL-C (Randox, UK), while serum triglyceride (TG) was determined by the methods of (Fossati and Prencipe 1982). Low density lipoproteins (LDL-C) concentration in the samples was determined by the methods of (Hoffmann et al. 1985), which was not significantly variant to those calculated with Friedwald’s equation (Friedewald et al. 1972).
3
Biochemical Profiling of Analytical Reagents
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All chemicals used in this study were of analytical grade and products of Sigma-Aldrich, St Louis, MO, USA. The triglyceride, cholesterol, calcium, alanine and aspartate transaminases and alkaline phosphatase, total protein, and albumin kits were products of Randox Laboratories Ltd, County Antrim, UK; the sodium and potassium kits were products of Atlas Medical, Cowley Rd, Cambridge, UK, while the chloride and magnesium kits were products of Agappe Diagnostics Switzerland GmbH.
4
Analysis of Medicinal Plant Compounds
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Fresh samples of the sclerotia of P. tuberregium were purchased from Mile 1 Market in Port Harcourt, Nigeria, while fresh leaves of C. aconitifolius (hospital too far) were collected from Farm Gardens in Aluu Community of Rivers State, Nigeria, and were duly identified as previously reported (Ikewuchi and Ikewuchi, 2009 , 2011 ; Ikewuchi et al., 2013a (link),b , 2014 (link), 2017 (link); Ifeanacho et al., 2019a (link),b , 2020 (link)). Ninety Wistar rats (weight 80–130 g) were obtained from the Animal House of the Department of Physiology, University of Port Harcourt, Nigeria. The alanine transaminase, aspartate transaminase, alkaline phosphatase, creatinine, and urea kits were the products of Randox Laboratories Ltd., County Antrim, UK.
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