To assess lymphocytes by flow cytometry, single cell suspensions were prepared and incubated with primary antibody for 30 minutes on ice. After staining for surface proteins, cells were incubated with Propidium Iodide (BD Biosciences, San Jose, CA, USA) for 10 minutes as a live/dead stain. After staining, cells were fixed with 0.6% formalin. The antibodies used were CD4-PE, CD5-PE, CD19-FITC, CD69-FITC, CD86-PE, B220-APC, CD93-BB515, CD279-APC, CXCR5-PECy7 (all BD Biosciences), IgM-FITC (Southern Biotech), IgD-APC-Cy7 (BioLegend, San Diego, CA, USA), CD21-eFlour450, and CD23-PE-Cy7 (eBioscience Inc., San Diego, CA, USA). Apoptosis was analyzed with Telford reagent. Flow cytometry was performed with a BD LSRII Flow Cytometer and analyzed with FACSDiva software (BD Biosciences, v.8.0).
Cd279 apc
Manufactured by BD
Sourced in United States
CD279-APC is a fluorescently labeled antibody that binds to the cell surface protein CD279, also known as Programmed Cell Death 1 (PD-1). It is commonly used in flow cytometry applications to detect and analyze the expression of PD-1 on various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Cd69 fitc, by BD (1 mentions)
Cd23 pe cy7, by Thermo Fisher Scientific (1 mentions)
Igd apc cy7, by BioLegend (1 mentions)
B220 apc, by BD (1 mentions)
Cd86 pe, by BD (1 mentions)
Cxcr5 pe cy7, by BD (1 mentions)
Igm fitc, by Southern Biotech (1 mentions)
Cd5 pe, by BD (1 mentions)
Cd4 pe, by BD (1 mentions)
Cd19 fitc, by BD (1 mentions)
Propidium iodide, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
Lsr 2 flow cytometer, by BD (1 mentions)
Coulter fc500 flow cytometer, by Beckman Coulter (1 mentions)
Cd152 pe, by BD (1 mentions)
Cd244 fitc, by BD (1 mentions)
Cd226 pe, by BD (1 mentions)
Interferon γ, by Thermo Fisher Scientific (1 mentions)
Cd274 pe, by BD (1 mentions)
Foxp3 pe, by BD (1 mentions)
2 protocols using cd279 apc
1
Comprehensive Lymphocyte Phenotyping by Flow Cytometry
2
Protocol for Generating CIK Cells
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Ten to fifteen milliliters of heparinized peripheral blood were collected for CIK in vitro expansion from health volunteers with informed consent. Peripheral blood mononuclear cells (PBMC) were separated by Ficoll density gradient centrifugation (GE Healthcare) and seeded at a concentration of 1.5×106 cells/ml in culture medium (Gibco, Life Technologies) with the addition of 1000 U/ml interferon-γ (Pepro Tech) and 50ng/ml anti-human OKT-3 (eBioscience) on day 0 and 300U/ml interleukin (IL)-2 (Huaxin High Biotechnology, Shanghai) on day 1. Phenotype and immune profile of CIK cells were characterized by multiparameter flow cytometric analysis using following monoclonal antibodies: CD3-FITC, CD56-PC7, CD3-PC7, CD4-FITC, CD8-FITC, CD314-PC7 (anti-NKG2D), CD152-PE (anti-CTLA-4), CD223-APC (anti-LAG-3), CD226-PE (anti-DNAM-1), CD244-FITC (anti-2B4), CD274-PE (anti-PD-L1), CD279-APC (anti-PD-1), Foxp3-PE (mAbs were from BD Biosciences). Flow cytometric analysis was performed using the Coulter FC500 flow cytometer (Beckman Coulter). Data were further analyzed by Flow Jo 7.6.5 software (Tree Star).
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