DMEM/F12 high glucose was provided by Hyclone (Utah, USA). Fatal bovine serum(FBS), trypsin, penicillin-streptomycin, Annix-V/PI kit, Cell Counting Kit-8(CCK-8), JC-1 kit and ROS kit were obtained from Gibco (New York, USA). Transwell chambers with membranes at a pore size of 8 μm were bought from Corning Life Science (New York, USA). Hoechst 33258 was purchased from Sigma-Aldrich (St Louis, MO, USA). PI was purchased from MultiSciences (Zhejiang Province, China). Primary antibodies against p53, p27, cleaved caspase-9, cleaved caspase-3, Bcl-2, Bax, cyclinD1, cyclin-dependent kinase 2 (CDK2), cytochrome c, apoptotic protease activating factor 1 (Apaf-1), LC3-I, LC3-II and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were provided by Cell Signaling Technology (Beverly, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies were provided by Huabio (Hangzhou, Zhejiang, China). Pifithrin-α (PFT-α) was provided by Selleck Chemicals (Shanghai, China). PNS was provided by Kunming Pharmaceutical Company (Kunming, China).
Apoptotic protease activating factor 1 apaf 1
Manufactured by Cell Signaling Technology
Sourced in United States
Apoptotic protease activating factor 1 (Apaf-1) is a protein that plays a central role in the intrinsic apoptosis pathway. It functions as a scaffold for the assembly of the apoptosome, a critical complex that activates the initiation of programmed cell death.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Cell counting kit 8 cck 8, by Thermo Fisher Scientific (2 mentions)
Hoechst 33258, by Merck Group (2 mentions)
Jc 1 kit, by Thermo Fisher Scientific (2 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
Cleaved caspase 9, by Cell Signaling Technology (2 mentions)
Cytochrome c, by Cell Signaling Technology (2 mentions)
Lc3 2, by Cell Signaling Technology (2 mentions)
Annix 5 pi kit, by Thermo Fisher Scientific (2 mentions)
Ros kit, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase hrp conjugated secondary antibodies, by Huabio (2 mentions)
Lc3 1, by Cell Signaling Technology (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fatal bovine serum, by Thermo Fisher Scientific (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Cyclin dependent kinase 2, by Cell Signaling Technology (1 mentions)
Pifithrin α pft α, by Selleck Chemicals (1 mentions)
Luteolin, by Merck Group (1 mentions)
Cell counting kit 8 (cck8), by Beyotime (1 mentions)
3 protocols using apoptotic protease activating factor 1 apaf 1
1
Assessing Apoptosis and Autophagy in Cells
2
Luteolin-Induced Cell Cycle Arrest and Apoptosis
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Luteolin was purchased from Sigma-Aldrich (EMD Millipore, Billerica, MA, USA); and was dissolved in dimethyl sulphoxide and its concentration was adjusted to 100 mmol/l, as a stock solution. The Cell Counting kit-8 was supplied by Beyotime Institute of Biotechnology (Haimen, China). Annexin V-fluorescein isothiocyanate (FITC) apoptosis and cell cycle detection kits were obtained from BD Biosciences (Franklin Lakes, NJ, USA). A bicinchoninic acid protein assay kit was purchased from Biosynthesis Biotechnology Co., Ltd. (Beijing, China) and monoclonal antibodies, including rabbit anti-human cell division cycle 2 (CDC2), cyclin-dependent kinase 2 (CDK2), cyclin B1, cyclin A, apoptotic protease activating factor 1 (APAF-1), cytochrome c, caspase-3, mouse anti-human procaspase-9, mouse anti-human caspase-9 and mouse anti-human β-actin, were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).
3
Apoptosis Pathway Regulation in Cells
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DMEM/F12 high glucose was provided by Hyclone (Utah, USA), fatal bovine serum(FBS), trypsin ,penicillin-streptomycin, Annix-V/PI kit, Cell Counting Kit-8(CCK-8), JC-1 kit and ROS kit were supplied by Gibco (New York, USA), transwell chambers with 8 μm-pore-sized membranes were bought from Corning Life Science (New York, USA), Hoechst 33258 was purchased from Sigma-Aldrich (St Louis, MO, USA), PI was purchased from MultiSciences (Zhejiang Province, China), primary antibodies against p53, p27,cleaved caspase-9, cleaved caspase-3, Bcl-2, Bax, cyclinD1, cyclin-dependent kinase 2 (CDK2), cytochrome c, apoptotic protease activating factor 1 (Apaf-1), LC3-I, LC3-II, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were provided by Cell Signaling Technology (Beverly, MA, USA), horseradish peroxidase (HRP)-conjugated secondary antibodies were provided by Huabio (Hangzhou, Zhejiang, China), pi thrin-α (PFT-α) was provided by Selleck Chemicals (Shanghai, China), PNS was provided by Kunming Pharmaceutical Company (Kunming, China).
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