The samples were then loaded onto 10% SDS–polyacrylamide gels. After transferring onto PVDF membranes, immunoblotting was performed using anti-Cdc2 (Santa Cruz Biotechnology, sc-53217) antibody, anti-phospho Cdc2 p34 (Tyr 15) (Santa Cruz Biotechnology, sc-7989) antibody, anti-phospho p38 [Cell Signaling Technology, 9211S; for phospho (p)-Sty1] or anti-Sty1 (lab stock) antibodies at 1:1000 dilution (or 1:2500 dilution for anti-Sty1). Immunoblots were developed using AdvanstaWesternBright™ ECL reagent (K-12045-D50).
Anti phospho cdc2 p34 tyr 15
Manufactured by Santa Cruz Biotechnology
Anti-phospho Cdc2 p34 (Tyr 15) is a lab equipment product that is used to detect the phosphorylation of Cdc2 p34 at tyrosine 15. It is a specific antibody that can be used in various immunological techniques to analyze the phosphorylation status of this important cell cycle regulator.
Automatically generated - may contain errors
2 protocols using anti phospho cdc2 p34 tyr 15
1
Immunoblotting Analysis of Cell Signaling
2
Immunoblotting Analysis of Cell Signaling
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The samples were then loaded onto 10% SDS–polyacrylamide gels. After transferring onto PVDF membranes, immunoblotting was performed using anti-Cdc2 (Santa Cruz Biotechnology, sc-53217) antibody, anti-phospho Cdc2 p34 (Tyr 15) (Santa Cruz Biotechnology, sc-7989) antibody, anti-phospho p38 [Cell Signaling Technology, 9211S; for phospho (p)-Sty1] or anti-Sty1 (lab stock) antibodies at 1:1000 dilution (or 1:2500 dilution for anti-Sty1). Immunoblots were developed using AdvanstaWesternBright™ ECL reagent (K-12045-D50).
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