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Hydroxyproline

Manufactured by HiMedia
Sourced in India

Hydroxyproline is a non-essential amino acid that is a component of collagen, the primary structural protein in connective tissues. It plays a crucial role in the stability and structural integrity of collagen fibrils.

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2 protocols using hydroxyproline

1

Antioxidant and Anti-inflammatory Assays

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Petroleum ether (60–80 °C), ethyl acetate, glacial acetic acid, sodium tartrate, copper sulfate, sodium carbonate, hydrochloric acid, chloroform, and perchloric acid were purchased from Ranbaxy Fine Chemicals Ltd., Thane, India. All these chemicals were of analytical grade. Folin-Ciocalteu reagent, Ehlrich reagent, 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were purchased from Sigma Chemical Co., Ltd., USA. Hydroxyproline, carrageenan, chloramine-T, and trichloracetic acid (TCA) were purchased from Hi Media Laboratories Pvt., Ltd., Mumbai, India.
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2

Antioxidant Assay Protocol

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Phosphate-buffered saline, hydroxyproline, Ehrlich’s reagent, and proteinase K were purchased from the HiMedia Laboratories (Mumbai, India). Silver nitrate, quercetin, and DPPH were purchased from Sigma-Aldrich (St. Louis, MI, USA).
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