Whole cell lysates were obtained using RIPA lysis buffer (Boster, AR0105-100). The samples were qualified using BC (Beijing full gold) quantitative determination. Proteins were separated on a 30% acrylamide gel (Amresco) and transferred to PVDF Transfer Membrane (Millipore). Subsequently, the membrane was blocked with 5% non-fat dry milk for 1 h at room temperature. After washing with TBST, the membrane was incubated overnight at 4 °C with the respective primary antibodies. The immunoblot was washed, diluted with TBST, and incubated at room temperature for 1 h. Finally, the protein bands were detected and photographed using a ChemiScope mini (Shanghai Qinxiang Scientific Instrument Co., Ltd.).
Acrylamide gel
Manufactured by Avantor
30% acrylamide gel is a laboratory equipment used for electrophoresis applications. It is a polyacrylamide gel solution with a concentration of 30% acrylamide. This gel is commonly used for the separation and analysis of proteins, nucleic acids, and other macromolecules.
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Lab products found in correlation
2 protocols using acrylamide gel
1
Western Blot Protein Analysis Protocol
2
Western Blot Protocol for Protein Characterization
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Whole cell lysates were obtained using RIPA lysis buffer (Boster, AR0105-100). The samples were quali ed by BCa (Beijing full gold) quantitative determination. Proteins were separated on a 30% acrylamide gel (Amresco) and transferred to PVDF Transfer Membrane (Millipore).It was then blocked with 5% non-fat dry milk for 1 hour at room temperature. The dilutions were washed with TBST and incubated overnight at 4°C. The immunoblot was washed, diluted with TBST, and incubated at room temperature for 1 h. Detected and photographed using a ChemiScope mini (Shanghai Qinxiang Scienti c Instrument Co., Ltd.).
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