Fenobam (30 mg/kg, i.p.; HelloBio, Princeton, NJ, USA) was dissolved in 100% dimethyl sulfoxide on the day of experiment in volumes of 20 μl7 (link). Behavioral experiments were conducted 5 minutes post-Fenobam injection. Gabapentin (30 mg/kg, i.p.; Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 0.9% saline on the day of experiment. Gabapentin was injected i.p. 1 hour prior to behavioral testing8 (link). Metformin (LKT Laboratories, St. Paul, MN, USA) and dissolved in 0.9% saline freshly for each use. Metformin was injected at 200 mg/kg daily for 7 consecutive days between 10am-1pm5 (link). Behavioral experiments were conducted at least 24 hours after the last Metformin injection. Carprofen (Zoetis, Kalamazoo, MI, USA) was administered i.p.,1 hour prior to behavioral testing at 50 mg/kg.
Metformin
Manufactured by LKT Laboratories
Sourced in United States
Metformin is a pharmaceutical product manufactured by LKT Laboratories. It is a white, crystalline powder that serves as an active pharmaceutical ingredient. Metformin is commonly used in the treatment of type 2 diabetes.
Automatically generated - may contain errors
2 protocols using metformin
1
Pharmacological Modulation of Behavior
2
Characterization of Nav1.7 in HEK293 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Nav1.7-transfected human embryonic kidney 293 (HEK293) cells were described previously [12 (link),36 (link),37 (link)]. Stock solutions of A769662 (LC Laboratories, Woburn, MA), Resveratrol (Cayman Chemicals, Ann Arbor, MI) and PT1 (Tocris, Minneapolis, MN) were made in DMSO and diluted in external solution bringing the final concentration of DMSO to <1%. For in vivo experiments, A769662 and PT1 were diluted in 100 mM PBS. Metformin was purchased from LKT Laboratories (St. Paul, MN) and stock solutions were made in double deionized water.
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