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Anti human ig

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Anti-human Ig is a laboratory reagent used to detect and quantify human immunoglobulins (Ig) in various biological samples. It functions as a detection tool for immunoassays and other analytical techniques.

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Lab products found in correlation

Streptavidin hrp, by Jackson ImmunoResearch (2 mentions) Aec substrate, by Merck Group (2 mentions) Anti human igm biotin, by Thermo Fisher Scientific (2 mentions) Igg igadouble color elispot kits, by Cellular Technology (2 mentions) Anelispot counter, by Cellular Technology (2 mentions) Elispot plate, by Merck Group (2 mentions) Cpg odn 2006, by InvivoGen (1 mentions) Anti human cd40, by Thermo Fisher Scientific (1 mentions) Human il 10 elisa kit, by Thermo Fisher Scientific (1 mentions) Anti human il 10 apc, by BioLegend (1 mentions) Golgiplug, by BD (1 mentions)

Spelling variants of this product

Anti-human Ig(A + G + M) F(ab′2) (3 citations) Goat-anti-human-Ig-APC (3 citations) Goat anti-human Ig (2 citations) Polyvalent goat anti-human Ig (2 citations)

3 protocols using anti human ig

1

Ex-vivo ELISpot for Antibody-Secreting Cells

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Direct ex-vivo ELISpot was performed to determine the
number of total, vaccine-binding, or recombinant HA-binding IgM-secreting cells
present in PBMC samples. ELISpot plates (Millipore) were coated overnight at
4°C with QIV (diluted 1:100), tetanus/diphtheria vaccine (diluted 1:50),
and 10 µg/mL anti-human Ig (Jackson ImmunoResearch) and 3 µg/mL
recombinant HA proteins. Plates were blocked the following morning for 90 min at
37°C with RPMI 1640 supplemented with 10% FBS. Dilutions of washed PBMCs
were incubated for 18 h in RPMI supplemented with 10% FBS. After washing the
plates, secreted antibodies were detected with anti-human IgM-biotin
(Invitrogen, 1:2000) and streptavidin-HRP (Jackson ImmunoResearch), and plates
were developed with AEC substrate (Sigma). Total, vaccine-binding, or
recombinant HA-binding IgG and IgA-secreting cells were detected using IgG/IgA
double -color ELISpot Kits (Cellular Technologies, Ltd.) according to the
manufacturer’s instructions. ELISpot plates were analyzed using an
ELISpot counter (Cellular Technologies Ltd.).
Turner J.S., Zhou J.Q., Han J., Schmitz A.J., Rizk A.A., Alsoussi W.B., Lei T., Amor M., McIntire K.M., Meade P., Strohmeier S., Brent R.I., Richey S.T., Haile A., Yang Y.R., Klebert M.K., Suessen T., Teefey S., Presti R.M., Krammer F., Kleinstein S.H., Ward A.B, & Ellebedy A.H. (2020). Human germinal centres engage memory and naïve B cells after influenza vaccination. Nature, 586(7827), 127-132.
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2

Ex-vivo ELISpot for Antibody-Secreting Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Direct ex-vivo ELISpot was performed to determine the
number of total, vaccine-binding, or recombinant HA-binding IgM-secreting cells
present in PBMC samples. ELISpot plates (Millipore) were coated overnight at
4°C with QIV (diluted 1:100), tetanus/diphtheria vaccine (diluted 1:50),
and 10 µg/mL anti-human Ig (Jackson ImmunoResearch) and 3 µg/mL
recombinant HA proteins. Plates were blocked the following morning for 90 min at
37°C with RPMI 1640 supplemented with 10% FBS. Dilutions of washed PBMCs
were incubated for 18 h in RPMI supplemented with 10% FBS. After washing the
plates, secreted antibodies were detected with anti-human IgM-biotin
(Invitrogen, 1:2000) and streptavidin-HRP (Jackson ImmunoResearch), and plates
were developed with AEC substrate (Sigma). Total, vaccine-binding, or
recombinant HA-binding IgG and IgA-secreting cells were detected using IgG/IgA
double -color ELISpot Kits (Cellular Technologies, Ltd.) according to the
manufacturer’s instructions. ELISpot plates were analyzed using an
ELISpot counter (Cellular Technologies Ltd.).
Turner J.S., Zhou J.Q., Han J., Schmitz A.J., Rizk A.A., Alsoussi W.B., Lei T., Amor M., McIntire K.M., Meade P., Strohmeier S., Brent R.I., Richey S.T., Haile A., Yang Y.R., Klebert M.K., Suessen T., Teefey S., Presti R.M., Krammer F., Kleinstein S.H., Ward A.B, & Ellebedy A.H. (2020). Human germinal centres engage memory and naïve B cells after influenza vaccination. Nature, 586(7827), 127-132.
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3

Intracellular IL-10 Profiling in MS B-cells

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For intracellular FACS of IL-10 in B-cell populations, we obtained fresh PBMCs from 5 IFN-β treated MS patients and 5 healthy volunteers and cultured at 2.5×106 cells/ml with 3ug of anti-human Ig (Jackson Immunoresearch), 1ug of anti-human CD40 (Ebioscience), 40nM CpG ODN 2006 (Invivogen), and Brefeldin A (GolgiPlug, BD Bioscience) in complete RPMI supplemented for 5 hrs then surface stained with anti-CD19 PerCP-Cy5.5, anti-CD24 FITC and anti-CD38 PE. Cells were then fixed, permeablized using the intracellular FACS kit (BD Bioscience) and stain with anti-human IL-10 APC (Biolegend). To assess secreted IL-10 by ELISA, fresh PBMCs (2.5×106 cells/ml) from 3 healthy volunteers were stimulated with or without anti-human Ig, anti-human CD40 and CpG in the presence or absence of 1000 units/ml of recombinant human IFN-β (PBL interferon source) for 72 hrs. IL-10 in culture supernatants were assessed by a Human IL-10 ELISA Kit (eBioscience).
Schubert R.D., Hu Y., Kumar G., Szeto S., Abraham P., Winderl J., Guthridge J.M., Pardo G., Dunn J., Steinman L, & Axtell R.C. (2015). Interferon-β treatment requires B cells for efficacy in neuro-autoimmunity.. Journal of immunology (Baltimore, Md. : 1950), 194(5), 2110-2116.
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