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2 protocols using phospho tau ser396

1

Tau Phosphorylation and Signaling Assays

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DHCR24 (#2033, Cell Signaling Technology), Tau5 (AHB0042, Thermo), Phospho-Tau (Thr181) (ab254409, Abcam), Phospho-Tau (Ser199) (#29,957, Cell Signaling Technology), Phospho-Tau (Ser396) (#9632, Cell Signaling Technology), Actin (#2118, Proteintech), cavin1 (#18,892–1-AP, Proteintech), Phospho-Tau (Ser262)(ab131354, Abcam), Phospho-Tau (Thr231)(#4137, ABclonal), Ras(27H5)(#3339, Cell Signaling Technology), p44/42 MAPK (ERK1/2) (137F5)(#4695S, Cell Signaling Technology), Phospho-p44/42 MAPK (ERK1/2)(Thr202/Tyr204)(#4730S, Cell Signaling Technology), MEK1/2 (D1A5)(#8727, Cell Signaling Technology), Phospho-MEK1/2 (Ser217/221)(#9154, Cell Signaling Technology), U0126 (#9903, Cell Signaling Technology), Propidium Iodide (abs9105, Absin), Methyl-β-cyclodextrin (abs42021762, Absin), Filipin III (abs42018484, Absin), Oil Red O (CAS:1320–06-5, O0625-25G, Sigma), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L)(SA00001-2, Proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L)(SA00001-1, Proteintech).
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2

Tau Protein Immunodetection Protocol

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Proteinase K (PK) and guanidine hydrochloride (GdnHCl) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Reagents for enhanced chemiluminescence (ECL Plus) were from Amersham Pharmacia Biotech, Inc. (Piscataway, NJ). Anti-tau mouse monoclonal antibodies RD3 and RD4 (Sigma-Aldrich) against human tau repeating region and sheep anti-mouse (SVM) IgG conjugated with horseradish peroxidase as a secondary antibody (AC111P, CHEMICON International, Inc, Burlington, MD) were used. Antibodies against Phospho-Tau (Thr231) and phospho-Tau (Ser396) were purchased from Cell Signaling Technology (Danvers, MA).
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