Rp c18 f254s

1D and 2D NMR spectra were obtained on a VARIAN UNITY INOVA 400 spectrometer (Varian, Palo Alto, CA, USA). Mass spectra were determined on a JEOL JMS-AX505WA mass spectrometer (Tokyo, Japan). Column chromatography was carried out over silica gel (40–60 μm, Merck, Kenilworth, NJ, USA) and LiChroprep RP-C18 (40–60 μm, Merck). HPLC (high performance liquid chromatography) was carried out on a Waters 1525 system (Miliford, MA, USA) using reverse-phase column (ODS-2, 5 μm, 4.6 × 15 cm, GL Science, Seoul, Korea). Fractions obtained from column chromatography were monitored by thin layer chromatography (TLC) (RP-C18 F_254S and silica gel 60 F₂₅₄, Merck).

Mass spectra were obtained on a JEOL JMS-AX505WA mass spectrometer. NMR spectra were determined on a Varian UNITY INOVA 400 NMR spectrometer. Column chromatography was carried out over silica gel (40–60 μm, Merck, Kenilworth, NJ, USA). Fractions from column chromatography were monitored by thin layer chromatography (TLC) (silica gel 60 F₂₅₄ and RP-C₁₈ F_254S, Merck) under UV light or by heating after spraying 10% H₂SO₄ in CH₃OH (v/v).

1D and 2D NMR spectra were recorded on an ECZ-400 spectrometer (Jeol Ltd., Tokyo, Japan). The signals of the solution CDCl₃ (residual CHCl₃ (δ_H 7.26 ppm) and CDCl₃ (δ_C 77.0 ppm)) were used as internal standards. NMR data were acquired and processed with Delta 6.1.0 or MestReNova v12.2.2 software. MS and HRMS spectra were acquired on an SolariX FTMS mass spectrometer (7 Tesla; Bruker, Bremen, Germany) with an ESI ion source in positive ionization mode. Silica gel (Kieselgel 60, 70–230 or 230–400 mesh, Merck, Darmstadt, Germany), and Sephadex LH-20 gel (Pharmacia Fine Chemicals AB, Uppsala, Sweden) were used for column chromatography (CC). Thin-layer chromatography (TLC) was carried out using silica gel (Kieselgel 60 F₂₅₄, Merck) and RP-C18 (F_254s, Merck) pre-coated plates. For visualization, the compounds were detected on TLC with a developer (50% H₂SO₄ (v/v) in methanol) followed by heating at 120 °C. HPLC analyses were performed with a pump (model L-2130, Hitachi, Tokyo, Japan) and a diode-array detector (model L-2455, Hitachi, Tokyo, Japan) equipped with an HPLC analytical column (BDS Hypersil^TM C18, 250 × 4.6 mm, 5 μm, ThermoFisher Scientific Inc., Waltham, MA, USA), and using a mixture of acetonitrile (ACN)/H₂O or a mixture of methanol (MeOH)/H₂O as the mobile phase (Supplementary Fig. 13).