D luciferin sodium salt

A stock solution of luciferase (product number: L9420), 1, 4-dithiothreitol (DTT), adenosine 5’-triphosphate disodium salt (ATP), coenzyme A sodium salt (CoA), BSA, ovalbumin (OVA), luminol sodium salt, 4-(imidazol-1-yl)phenol (4-IMP), hydrogen peroxide solution (H₂O₂), peroxidase from horseradish (HRP) and thrombin were purchased from Sigma-Aldrich (St. Louis, MO, USA). D-luciferin sodium salt was purchased from J&K Scientific (Beijing, China). Collagen was purchased from Hyphen Biomed (Neuville-sur-Oise, France). Hank’s balanced salt solution (HBSS) was obtained from ThermoFisher Scientific (Waltham, MA, USA). Ly294002 (phosphoinositite 3 kinase (PI3K) inhibitor) and Staurosporine (protein kinase C (PKC) inhibitor) were purchased from Abmole Bioscience (Houston, TX, USA). Prostaglandin E1 (PGE1) and other commonly used chemical reagents were purchased from Aladdin Industrial (Shanghai, China).

CuS@BSA and PLA electrospinning film was previously synthesized by our group. Primary antibody vimentin (#5741), β-actin (#3700), ERP/P-ERK (#9926), second antibody conjugated Alexa Fluor^® 488(#4408) and Alexa Fluor^® 647 (#4418) were purchased from Cell signaling technology company. The second antibody conjugated HRP (goat-anti-mouse and goat-anti-rabbit) were purchased from Proteintech. Cultrex^® Reduced Growth Factor Basement Membrane Extract (#3533-005-02) was purchased from Trevigen.
Tegaderm film was purchased from 3M Health Care. Puromycin (A1113803) was purchased from Thermo Fisher Scientific. D-Luciferin sodium salt (308290) was purchased from J&K Scientific.
Male Sprague-Dawley (SD) albino rats and BALB/c mice were purchased from the Laboratory Animal Center of Sichuan University. All the rats were housed individually to prevent fighting and attack to the wounds, and received food and water ad libitum. All the animals were quarantined for a week before treatment. All animal procedures were performed following the protocol approved by the Institutional Animal Care and Treatment Committee of Sichuan University (Chengdu, P.R. China). All animals were treated humanely throughout the experimental period.