Blood lead (BPb) level was measured using Varian AA240 Atomic Absorption Spectrophotometer according to the method described by the American Public Health Association.[9 ] Serum total cholesterol (TC) was determined by enzymatic colorimetric method as described by Grove.[10 (link)] Serum triglyceride (TG) was estimated by enzymatic colorimetric reaction according to the method described by Fossati and Recipe;[11 (link)] low-density lipoprotein cholesterol (LDL-C), and very low-density lipoprotein cholesterol (VLDL-C) were calculated based on Friedewald's formula.[12 (link)]
Aa240 atomic absorption spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States
The AA240 Atomic Absorption Spectrophotometer is a laboratory instrument designed for the quantitative determination of trace elements in a variety of sample matrices. It utilizes the principle of atomic absorption, where atoms in the sample are excited by a light source, and the amount of light absorbed is measured to determine the concentration of the target element.
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4 protocols using aa240 atomic absorption spectrophotometer
1
Measuring Blood Lead and Lipid Profiles
2
Heavy Metal Analysis Protocol
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Heavy metal contents were determined using Varian AA240 Atomic Absorption Spectrophotometer according to the methods described by American Public Health Association [18] .
3
Atomic Absorption Spectroscopy for Mineral Analysis
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Laboratory analysis: The mineral compositions were determined using Varian AA240 Atomic absorption spectrophotometer based on quadrupole mass analyser and octapole reaction system. (FS240AA Agilent Atomic absorption spectroscopy, USA make) [17] . Collision cell in He-mode was used for elimination of possible polyatomic interferences and instrument was set up by using Tuning solution (Agilent Technologies, Santa Clara, USA).
Data quality: Data quality (18) was confirmed used laid out gold standard to increase the overall reliability, reproducibility, and specificity of data generated for analysis in this study. To be more specific, the calibration solutions were prepared by the appropriate dilution of the single element certified reference materials. Measurement accuracy was verified by using certified reference material of water as was optimized by the Springboard research laboratories. Awka, Anambra State, Nigeria where the samples were analysed.
Data quality: Data quality (18) was confirmed used laid out gold standard to increase the overall reliability, reproducibility, and specificity of data generated for analysis in this study. To be more specific, the calibration solutions were prepared by the appropriate dilution of the single element certified reference materials. Measurement accuracy was verified by using certified reference material of water as was optimized by the Springboard research laboratories. Awka, Anambra State, Nigeria where the samples were analysed.
4
Soil and Plant Heavy Metal Analysis
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All reagents were of analytical grade: HNO 3 (65 %; Carlo Erba, Rodano, Italy), H 2 O 2 (30 %; Belinka Perkemija, Ljubljana, Slovenia), HCl (37 %; Sigma Aldrich, Steinheim, Germany), ethanol (96 %; Scharlau Chemie S.A., Barcelona, Spain), NH 4 OAc (Fluka, Steinheim, Germany). High-purity water (electrical resistivity >10 MΩcm) was produced with a Milli-Q system (Millipore, Bedford, MA, USA). Solutions were made using demineralised water (electrical resistivity 18.2 MΩcm, toc<10 µg L -1 ) produced with the Milli-Q system. Calibrated solutions were prepared from 1 g L -1 stock solutions (Merck, Steinheim, Germany).
The Cd and Pb content in all soil and plant extracts was determined with a Perkin Elmer AAnalyst 600 atomic absorption spectrophotometer with a HGA 400 graphite furnace (Waltham, MA, USA) equipped with pyrolytic graphite tubes and temperature programmes that are presented elsewhere (15, (link)16) . The concentrations of Fe, Mn, Zn, and Cu were determined with a Varian AA240 atomic absorption spectrophotometer (Palo Alto, CA, USA) under optimal conditions using suitable hollow cathode lamps. Signals were measured with a background correction (deuterium lamp) at the optimal flame (A-Ac) height (15, (link)16) .
The Cd and Pb content in all soil and plant extracts was determined with a Perkin Elmer AAnalyst 600 atomic absorption spectrophotometer with a HGA 400 graphite furnace (Waltham, MA, USA) equipped with pyrolytic graphite tubes and temperature programmes that are presented elsewhere (15, (link)16) . The concentrations of Fe, Mn, Zn, and Cu were determined with a Varian AA240 atomic absorption spectrophotometer (Palo Alto, CA, USA) under optimal conditions using suitable hollow cathode lamps. Signals were measured with a background correction (deuterium lamp) at the optimal flame (A-Ac) height (15, (link)16) .
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