Amicon ultra 0.5 30k mwco centrifugal filter device

Manufactured by Merck Group

Sourced in Germany

The Amicon Ultra-0.5/30K MWCO Centrifugal Filter Device is a laboratory equipment used for sample concentration and buffer exchange. It features a 30 kDa molecular weight cut-off (MWCO) membrane that allows the passage of molecules smaller than 30 kDa while retaining larger molecules during centrifugation.

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Lab products found in correlation

Slide a lyzer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Amicon centrifugal filters (166 citations) Amicon Ultra filters (132 citations) Centrifugal filter devices (50 citations) Amicon Ultra 30K (31 citations) Amicon Ultra device (23 citations) Amicon centrifugal devices (20 citations) MWCO centrifugal filter (11 citations) Amicon Ultra-0.5 30K (4 citations) 30K Amicon centrifugal filters (2 citations) Amicon filter devices (2 citations)

2 protocols using amicon ultra 0.5 30k mwco centrifugal filter device

AAV Vector Buffer Exchange and Concentration

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The purified vector, at a concentration of 2e12 to 5e12 vg/mL, was buffer exchanged into PBS (pH 7.2) using a 10K MWCO Slide-a-Lyzer (Thermo Scientific, Waltham, MA, USA). The AAV vector absorbance signal was determined by optical density measurement at 260 nm (OD₂₆₀) using spectrophotometric methods. For consistency, the samples were adjusted to a target concentration (OD₂₆₀ of between 0.2 and 0.8) either by direct dilution with PBS or further concentrated using an Amicon Ultra-0.5/30K MWCO Centrifugal Filter Device (Merck, Darmstadt, Germany).

Nass S.A., Mattingly M.A., Woodcock D.A., Burnham B.L., Ardinger J.A., Osmond S.E., Frederick A.M., Scaria A., Cheng S.H, & O’Riordan C.R. (2017). Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes. Molecular Therapy. Methods & Clinical Development, 9, 33-46.

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AAV Vector Purification and Concentration

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Purified vector, at a concentration of 2.0 × 10¹²–5.0 × 10¹² vg/mL, was buffer exchanged into phosphate-buffered saline (PBS), pH 7.2, using a 10,000 MWCO Slide-a-Lyzer (Thermo Scientific, Waltham, MA). The AAV vector absorbance signal was determined by optical density 260 (OD₂₆₀) using spectrophotometric methods. For consistency, the samples were adjusted to a target concentration (OD₂₆₀ of between 0.2 and 0.8) by direct dilution with PBS or further concentrated using an Amicon Ultra-0.5/30K MWCO Centrifugal Filter Device (Merck, Darmstadt, Germany).

Boye S.L., O’Riordan C., Morris J., Lukason M., Compton D., Baek R., Elmore D.M., Peterson J.J., Fajardo D., McCullough K.T., Scaria A., McVie-Wylie A, & Boye S.E. (2022). Preclinical studies in support of phase I/II clinical trials to treat GUCY2D-associated Leber congenital amaurosis. Molecular Therapy. Methods & Clinical Development, 28, 129-145.

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