Anti-histone H3 antibody (4499) and peroxidase-conjugated anti-rabbit antibody (7074) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-Nrf2 and anti-Keap1 antibodies have been described previously [24 (link),25 (link)]. Peroxidase-conjugated anti-mouse antibody (NA931) was purchased from GE Healthcare (Piscataway, NJ, USA). Anti-Nqo1 antibody (ab2346) was purchased from Abcam (Cambridge, UK). Other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise specified.
Anti histone h3 antibody 4499
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-histone H3 antibody (4499) is a laboratory reagent used to detect the presence of histone H3, a core histone protein found in eukaryotic cells. This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunocytochemistry, to study the expression and localization of histone H3 in biological samples.
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2 protocols using anti histone h3 antibody 4499
1
Antibody-based protein detection protocol
2
Molecular Mechanisms of Inflammasome Activation
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Bilirubin (B4126), ethidium bromide (EtBr) (E1385), and 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) (M5655) were purchased from Sigma-Aldrich, Co. (St. Louis, MO). Ethidium homodimer-2 (EthD2) (E3599) was purchased from Life Technologies (Eugene, OR). VX-765 (S2228) was purchased from Selleck Chemicals (Houston, TX). The Caspase-Glo 1 assay was purchased from Promega (Madison, WI). Anti-Gasdermin D (anti-GSDMD) antibody (93709), anti-nuclear factor-kappa B (NF-κB) p65 antibody (8242), anti-Histone H3 antibody (4499), and anti-β-actin (3700) were purchased from Cell Signaling Technology (Danvers, MA). Anti-NeuN antibody (ab177487) was purchased from Abcam (Cambridge, UK). The nonradioactive infrared NF-κB electrophoretic mobility shift assay (EMSA) Kit was purchased from Viagen Biotech (Changzhou, China). The enzyme-linked immunosorbent assay (ELISA) kits for IL-1β, IL-18, IL-6, and tumor necrosis factor (TNF)-α were purchased from Raybiotech (Norcross, GA). Quanti-Nova SYBR Green PCR Kit (208054) was purchased from Qiagen (Hilden, Germany).
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