Human GC cell lines AGS, NCI-N87, BGC-823, HGC-27, KATO III, SGC-7901, SNU-1, SNU-5, and SNU-16 were purchased from American Type Culture Collection (ATCC). AGS, BGC-823, HGC-27, KATO III, and SNU-5 cells were cultured in DMEM-GlutaMAX medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS; Life Technologies), penicillin (100 U/ml), and streptomycin (100 μg/ml; Life technologies). NCI-N87, SGC-7901, SNU-1, and SNU-16 cells were cultured in RPMI 1640-GlutaMAX medium (Life Technologies) supplemented with 10% FBS, penicillin (100 U/ml), and streptomycin (100 μg/ml). All the cells were maintained in a 5% CO2 humidified atmosphere at 37°C. The ATG5 and ATG7 siRNAs were purchased from Life Technologies. CQ, cobalt chloride (CoCl2) and Sp600125 were purchased from Sigma. Anisomycin was purchased from Cell Signaling Technology. Recombinant CXCL10 protein, CXCL10 antibody and mouse IgG1 isotype control were purchased from R&D systems. The plasmid pIREShyg3 was purchased from GenScript and the coding sequence (CDS) of CXCL10 gene was cloned in pIREShyg3 using Nhel / BamHI to obtain the pIREShyg3-CXCL10 plasmid.
Meng Q., Zhang Y, & Hu L.G. (2020). Targeting Autophagy Facilitates T Lymphocyte Migration by Inducing the Expression of CXCL10 in Gastric Cancer Cell Lines. Frontiers in Oncology, 10, 886.
+ Open protocol