600 e pump controller

Calibration curves were constructed by spiking acyclovir in the blank AH and cornea. Acyclovir from the tissue was analyzed using a Waters HPLC system with a 600 E pump controller, 717 plus autosampler, and 2486 UV detector. A Phenomenex Luna® 5 μm C18 100 A° 250 × 4.60 mm column was used with a mobile phase that consisted of acetonitrile:water (1:9, v/v) at a flow rate of 0.75 mL/min. The detection wavelength was set at 254 nm and 20 μL of each sample was injected into the column. Final concentrations of the standard solutions were in the range of 10–400 ng/mL and the constructed standard curve had a coefficient of determination (R²) > 0.941.

Valacyclovir hydrochloride was quantified using a Waters HPLC system with 600 E pump controller, 717 plus autosampler, and 2486 UV detector. The in vitro release study, drug content and the trans-corneal samples were analyzed using the Phenomenex Luna® 5 μm C18 100 A° 250 × 4.60 mm column at a flow rate of 0.75 mL/min. The mobile phase used for the analysis of valacyclovir hydrochloride was a methanol and 0.1% aqueous solution of glacial acetic acid (1:9, v/v) at 254 nm with injection volume of 20 μL. All experiments were carried out in triplicate.