Levels of the cytokines IFN-α (ExCell Biology, China) and IFN-β (CUSABIO, China) in the culture supernatant of Hepa1-6 cells and in the serum of tumor-bearing mice were detected by Enzyme-linked immunosorbent assay (ELISA). Concentrations of the cytokines TGF-β, and IL-10 (Multi Sciences Biotech, China) in the serum or tumor tissue homogenate of tumor-bearing mice were also detected by ELISA. ELISAs for cytokine detection were performed according to the manufacturer’s instructions.
Ifn β
Manufactured by Cusabio
Sourced in China
IFN-β is a laboratory product used for research purposes. It is a type of interferon beta protein that plays a role in the immune system. The core function of IFN-β is to induce an antiviral state in cells, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
3 protocols using ifn β
1
Quantifying Tumor Immunomodulatory Cytokines
2
ELISA Quantification of IFN-α and IFN-β
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Serum levels of IFN-α (ExCell Biology, Shanghai, China) and IFN-β (CUSABIO, Wuhan, China) were detected by ELISA according to the manufacturer's instructions.
3
Quantification of Inflammatory Cytokines
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Serum and BALF were prepared as described above. Levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-10 (R&D Systems, Inc., Minneapolis, MN), and interferon β (IFN-β) (Cusabio, Wuhan, China) in BALF and serum were measured by commercially available enzyme-linked immunosorbent assay (ELISA) kits for rat, following the manufacturer’s instructions. In brief, diluted standards or samples were added to 96-well plates precoated with affinity purified polyclonal antibodies specific for TNF-α, IL-1β, IL-10, and IFN-β, respectively. Enzyme-linked polyclonal antibodies were then added to the wells prior to incubation at 37 °C for 60 min, followed by five final washes. The intensities detected at 450 nm were measured after the addition of substrate solutions for 15 min. Each sample was assayed in triplicate. Levels of TNF-α, IL-1β, IL-10, and IFN-β were calculated according to standard curves.
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