Glutamine supplement

Manufactured by Thermo Fisher Scientific

Sourced in United States

Glutamine supplement is a laboratory product that provides a source of the amino acid glutamine. Glutamine is a key component of cellular metabolism and plays a role in various biological processes. The supplement is intended to support research and scientific experimentation in relevant fields.

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Lab products found in correlation

Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions) 35 mm dishes, by Corning (1 mentions) Poly d lysine pre coated 96 well plates, by Corning (1 mentions)

Close spelling variants of this product

Glutamine (2902 citations) L-glutamine and B-27 supplement (5 citations) L-Glutamine supplement (2 citations) Penicillin-Streptomycin-Glutamine supplement (2 citations) GlutaMAX L-glutamine supplement (2 citations) N2 supplement andl-glutamine (2 citations)

2 protocols using glutamine supplement

Embryoid Body Formation Protocol

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EB formation was performed according to a recent report [33 ]. In brief, 5 × 10⁵ ESCs were resuspend in mTesR1 medium with 10 μm Y-27632 and then plated on 6-well low attachment plates at 37 °C with 5% CO₂ incubator to allow cells self-aggregation. After 24 h, the small EBs were transferred to a new low attachment plate and these aggregates were cultured in 3 ml EB medium (DMEM containing 20% knockout serum replacement (KSR, Gibco, USA, Cat#10828028), 1x glutamine supplement (Gibco, USA, Cat#35050079), 1% NEAA (Gibco, USA, Cat#11140050), and 0.2% β-Mercaptoethanol (Gibco, USA, Cat#21985023)). The EB medium was changed every 2 days. On day 7, the EBs were collected for gene expression analysis.

Ding S., Lan X., Meng Y., Yan C., Li M., Li X., Chen J, & Jiang W. (2021). CHD8 safeguards early neuroectoderm differentiation in human ESCs and protects from apoptosis during neurogenesis. Cell Death & Disease, 12(11), 981.

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Embryonic Mouse Cortical Neuron Culture

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Brains were removed from embryonic day 15 C57BL/6 mice [36 (link)] and maintained in high glucose Dulbecco's Modified Eagle Medium (DMEM) with 25μM of 2-mercaptoethanol at 4°C during dissection. Cortices were dissected and dissociated in trypsin (0.05% with EDTA) and DNAse (10 μg/ml) and then resuspended in neurobasal (NB) medium with 2% B27 and 1mM glutamine supplement (Gibco, Rockville, MD). The cells were plated onto poly-D-lysine precoated 96-well plates or 35-mm dishes (Corning Life Sciences, Tewksbury, MA) at a density of 1 × 10⁵ cells/cm². The cultures were kept at 37°C in a humidified incubator with a 5% CO₂ atmosphere. Three days after plating, half of the medium was replaced with the NB medium with 5-Fluoro-2’- deoxyuridine (4 μg/ml) and Uridine (20 μg/ml) to inhibit growth of non-neuronal cells. The neurons were used on 7–8 day in vitro (DIV) for oxygen-glucose deprivation (OGD) experiments.

Liang X., Liu X., Lu F., Zhang Y., Jiang X, & Ferriero D.M. (2019). HIF1α signaling in the endogenous protective responses after neonatal brain hypoxia-ischemia. Developmental neuroscience.

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