Fam mgb taqman system

Manufactured by Thermo Fisher Scientific

The FAM–MGB TaqMan system is a real-time PCR detection system that utilizes dual-labeled fluorogenic probes for the detection and quantification of specific DNA sequences. The system employs the 5′ nuclease activity of Taq DNA polymerase and a minor groove binder (MGB) moiety to enhance probe binding and specificity. This technology provides a sensitive and reliable method for performing real-time PCR analysis.

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Lab products found in correlation

High capacity rna to cdna kit, by Thermo Fisher Scientific (2 mentions) 7500 fast real time pcr system, by Thermo Fisher Scientific (2 mentions) Rneasy mini kit, by Qiagen (1 mentions) Allprep dna rna universal kit, by Qiagen (1 mentions)

2 protocols using fam mgb taqman system

RNA Extraction and RT-PCR Analysis

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RNA was extracted from cell and tissue samples using RNeasy Mini Kits according to the manufacturer’s instructions (Qiagen). cDNA was generated from RNA using a High-Capacity RNA to cDNA Kit (Applied Biosystems). Each RT-PCR reaction utilized 25 ng cDNA and was analyzed in technical duplicates or triplicates. Reactions were analyzed on a 7500 Fast Real-Time PCR System (Applied Biosystems). All primer/probes used in this study utilized the FAM–MGB TaqMan system and were purchased from Applied Biosystems or were generated using the primer design tool Primer-BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast) (Suppl. Table S1, Online Resource 1).

Reyes J.F., Sackmann C., Hoffmann A., Svenningsson P., Winkler J., Ingelsson M, & Hallbeck M. (2019). Binding of α-synuclein oligomers to Cx32 facilitates protein uptake and transfer in neurons and oligodendrocytes. Acta Neuropathologica, 138(1), 23-47.

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Quantitative RT-PCR Analysis of Gene Expression

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RNA was extracted from cell and tissue samples using AllPrep DNA/RNA Universal Kit according to the manufacturer’s instructions (Qiagen). cDNA was generated from RNA using a High- Capacity RNA to cDNA Kit (Applied Biosystems).
Each RT-PCR reaction utilized 10 ng cDNA and was analyzed in technical duplicates. Reactions were analyzed on a 7500 Fast Real-Time PCR System (Applied Biosystems). All primer/probes used in this study utilized the FAM–MGB TaqMan system and were purchased from Applied Biosystems or were generated using the primer design tool Primer-BLAST (http://www.ncbi.nlm.nih.gov/ tools/primer-blast) (Additional file 10: Table I). Amplification of GAPDH was used as an internal standard. The Ct method was used to determine the fold-difference in expression levels relative to a control sample.

Reyes J.F., Ekmark-Léwen S., Perdiki M., Klingstedt T., Hoffmann A., Wiechec E., Nilsson P., Nilsson K.P., Alafuzoff I., Ingelsson M, & Hallbeck M. (2021). Accumulation of alpha-synuclein within the liver, potential role in the clearance of brain pathology associated with Parkinson’s disease. Acta Neuropathologica Communications, 9, 46.

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