HUVEC׳s were purchased from PromoCell (Germany) and were used between passages 3 and 5. Cells were cultured in Complete Endothelial Cell Growth Media (EGM) (PromoCell, Germany), supplemented with 10% FCS (FirstLink,UK) and 1% Penicillin/Streptomycin (Gibco,UK). HBMSCs were obtained from patients at the RNOH undergoing total hip replacements (with informed consent and ethical approval) and were isolated based on the method by Igarashi et al. [20] (link). Cells were cultured in low glucose Dulbecco׳s Modified Eagles Medium (DMEM, Sigma USA) supplemented with 20% FCS and 1% Penicillin/Streptomycin. Cells were detached from tissue culture flasks, following washes with Phosphate Buffered Saline (PBS), and incubation with 0.5% trypsin at 37 °C for 5 min.
HUVEC׳s were pre-tested by PromoCell for cell proliferation and morphology. These were also positive for EC specific markers such as CD31 and von Willebrand Factor. HBMSCs were CD31 negative cells, with mesenchymal cell characteristics and differentiation potential into osteoblasts, chondrocytes and adipocytes.
HUVEC׳s were pre-tested by PromoCell for cell proliferation and morphology. These were also positive for EC specific markers such as CD31 and von Willebrand Factor. HBMSCs were CD31 negative cells, with mesenchymal cell characteristics and differentiation potential into osteoblasts, chondrocytes and adipocytes.