Phospho tbk1 ser172

Manufactured by Abcam

Sourced in United States

Phospho-TBK1 (Ser172) is a lab equipment product that detects the phosphorylation of TBK1 at serine 172. It is used to study the activation of the TBK1 protein, which plays a role in innate immune signaling pathways.

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Lab products found in correlation

Streptavidin agarose, by Thermo Fisher Scientific (1 mentions) Flag m2 antibody, by Merck Group (1 mentions) Phospho irf3 ser396, by Cell Signaling Technology (1 mentions) Poly 1 c, by InvivoGen (1 mentions) αp32 atp, by PerkinElmer (1 mentions) Tbk1 antibody, by Abcam (1 mentions) Gapdh 6c5, by Santa Cruz Biotechnology (1 mentions) Nu7026, by MedChemExpress (1 mentions) Vdac1 b 6, by Santa Cruz Biotechnology (1 mentions) Phospho histone h2a x ser139 antibody, by Merck Group (1 mentions) Herring testis dna, by Merck Group (1 mentions) Cgas d1d3g, by Cell Signaling Technology (1 mentions) Nu7441, by Selleck Chemicals (1 mentions) 2 3 cgamp, by InvivoGen (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) β actin, by Abcam (1 mentions) Hrp conjugated goat anti rabbit, by Cell Signaling Technology (1 mentions) Phospho irf3, by Cell Signaling Technology (1 mentions) Rela p65 nf κb, by Cell Signaling Technology (1 mentions) Gal 1, by Abcam (1 mentions)

2 protocols using phospho tbk1 ser172

Antibody Validation and Phosphorylation Assays

Cited 1 time

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Commercially available antibodies used for this study include mouse monoclonal FLAG M2 antibody (1:5000) (Sigma); rabbit polyclonal V5 antibody (1:5000) (Thermo Fisher); mouse monoclonal V5 (1:5000), β-actin (1:5000), and TBK1 antibody (1:1000) (Abcam); Phospho-TBK1 (Ser172) (1:1000), Phospho-IRF3 (Ser396) (1:1000), Ku80 (1:1000), cGAS (D1D3G) (1:1000), and STING (D2P2F) antibody (1:1000) (Cell Signaling); GAPDH (6C5) (1:1000), VDAC1 (B-6) (1:1000), and IRF3 antibody (FL-425) (1:1000) (Santa Cruz); DNA-PKcs (1:1000) and Ku70 antibody (1:1000) (NeoMarkers); and phospho-Histone H2A.X (Ser139) antibody (1:1000) (Millipore).
Rabbit polyclonal anti-cGAS pT68 and pS213 antibodies were generated by GenScript Company (NJ, USA) and used at 1:500 dilution. Rabbit polyclonal anti-MAVS antibody was generated in the laboratory and used at 1:1000 dilution^{48 (link)}.
Nu7441 (Selleck Chemical), Nu7026 (MedChemExpress), Herring testis (HT)-DNA (Sigma-Aldrich), poly I:C (InvivoGen), 2’,3’-cGAMP (InvivoGen), streptavidin agarose (Thermo Fisher), Ni-NTA His-Bind Resin (Novagen), and Malachite Green Phosphate Detection Kit (Cell Signaling) were used according to the manufacturer’s protocol. Biotin-labeled ISD-45 DNA was ordered from IDT. [α-P32]-ATP was ordered from Perkin Elmer. Lipofectamine 2000 was purchased from Invitrogen.

Sun X., Liu T., Zhao J., Xia H., Xie J., Guo Y., Zhong L., Li M., Yang Q., Peng C., Rouvet I., Belot A., Shu H.B., Feng P, & Zhang J. (2020). DNA-PK deficiency potentiates cGAS-mediated antiviral innate immunity. Nature Communications, 11, 6182.

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Protein Analysis Protocol for STING Pathway

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For protein analysis, cells were lysed in RIPA lysis buffer and processed for protein loading as previously published[9 (link)]. The following primary antibodies were used to detect specific proteins: Gal1 (Cat: ab138513, Abcam), phospho-STING (Cat: 72971), phospho-TBK1 (ser172, Cat: 5483), STING (Cat:13647), TBK1 (Cat: 3504), IRF-3 (Cat: 4302, RRID: AB_1904036), phospho-IRF-3 (Cat: 29047, RRID: AB_2773013), RelA/p65 (NF-κB (Cat: 8242), phospho-NF-κB (RelA/p65, Cat:3033), NF-κB1 p105/p50 (Cat: 3035), NF-κB2 p100/p52 (Cat: 4882) from Cell Signaling Technologies, and β-Actin (1:5000; Cat: sc-47778 HRP, Santa Cruz Biotechnologies). Secondary antibodies used in this study were HRP-conjugated Donkey anti-Goat IgG (H+L) (1:5000-1:10,000; Cat: A15999 Invitrogen), HRP-conjugated goat anti-rabbit (1:5000; Cat: 7074 Cell Signaling Technologies). Immunoblots were developed with Pierce West Pico (Cat: 35060; Thermo Fisher Scientific) and visualized with ChemiDoc XRS imaging system equipped with Image Lab Software (RRID: SCR_008426; Bio-Rad Laboratories). In each case, experiments were carried out in triplicate and a representative blot is shown unless otherwise stated. Blot densitometry quantification was done using ImageJ (RRID: SCR_003070)

Rørvik L.M., Aase B., Alvestad T, & Caugant D.A. (2000). Molecular Epidemiological Survey of Listeria monocytogenes in Seafoods and Seafood-Processing Plants. Applied and Environmental Microbiology, 66(11), 4779-4784.

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