Anti u2af65

Manufactured by Santa Cruz Biotechnology

Anti-U2AF65 is a laboratory reagent used in molecular biology research. It is an antibody that specifically binds to the U2AF65 protein, which is a component of the spliceosome involved in pre-mRNA splicing. The core function of Anti-U2AF65 is to detect and quantify the presence of U2AF65 in biological samples.

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Lab products found in correlation

Anti yb 1, by Abcam (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Protein a agarose bead, by Roche (1 mentions) Ab5095, by Abcam (1 mentions) Anti vinculin, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti cdk9, by Santa Cruz Biotechnology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti hnrnp f h, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti rl2, by Santa Cruz Biotechnology (1 mentions) Anti u1a, by Thermo Fisher Scientific (1 mentions) Anti u1 70k, by Synaptic Systems (1 mentions) Anti srsf1, by Thermo Fisher Scientific (1 mentions) Anti rbfox2, by Fortis Life Sciences (1 mentions) Anti o glcnac ctd110.6, by Cell Signaling Technology (1 mentions) Anti gfpt1, by Abcam (1 mentions) Alexa 488 conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Anti gapdh, by Merck Group (1 mentions)

Close spelling variants of this product

U2AF65 (9 citations)

3 protocols using anti u2af65

Co-immunoprecipitation of Protein Complexes

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Co-immunoprecipitation of ILF2, YB-1 or U2AF65 complexes was performed by lysing JJN3 cells with NP40 lysis buffer containing phosphatase and protease inhibitor cocktails (Roche) on ice for 1 hr. Lysates were sonicated gently and centrifuged for 10 min at 4°C. One milligram of the lysate was incubated overnight at 4°C with Protein A-agarose beads (Roche) and anti-ILF2 (Abcam, ab113205), anti–YB-1 (Abcam, ab12148), anti-U2AF65 (Santa Cruz, sc53942) -or unspecific (rabbit/mouse immunoglobulin G [IgG], Santa Cruz, sc-2027/2025) antibodies for immunoprecipitation. The immunoprecipitates were then re-suspended in sample buffer, boiled for 5 min, and analyzed by Western blotting or mass spectrometry.

Marchesini M., Ogoti Y., Fiorini E., Samur A.A., Nezi L., D'Anca M., Storti P., Samur M.K., Ganan-Gomez I., Fulciniti M.T., Mistry N., Jiang S., Bao N., Marchica V., Neri A., Bueso-Ramos C., Wu C.J., Zhang L., Liang H., Peng X., Giuliani N., Draetta G., Clise-Dwyer K., Kantarjian H., Munshi N., Orlowski R., Garcia-Manero G., DePinho R.A, & Colla S. (2017). ILF2 Is a Regulator of RNA Splicing and DNA Damage Response in 1q21-Amplified Multiple Myeloma. Cancer cell, 32(1), 88-100.e6.

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Antibodies for ChIP and Western Blotting

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The following antibodies were used for chromatin immunoprecipitation (ChIP) or western blotting (WB): anti-PFDN5 (Santa Cruz, sc-271150, WB); anti-GAPDH (Santa Cruz, sc-47724, WB); anti-PFDN2 (Bethyl, A304-807A, WB); anti-Rpb1 CTD Ser2 phosphorylated (Abcam, ab5095, ChIP and WB); anti-Rpb1 CTD Ser5 phosphorylated (Abcam, ab5131, ChIP and WB); anti-Rpb1 8WG16 (Thermo Fisher MA1-10882, ChIP); anti-FLAG (Sigma, F3165, ChIP); anti-U2AF65 (Santa Cruz, sc-53942, ChIP and WB); anti-PRPF19 (Lührmann Laboratory, ChIP and WB); anti-PRGL1 (Lührmann Laboratory, WB); anti-CDK9 (Santa Cruz, sc-13130, ChIP and WB); anti-vinculin (Santa Cruz, sc-25336, WB).

Payán-Bravo L., Fontalva S., Peñate X., Cases I., Guerrero-Martínez J.A., Pareja-Sánchez Y., Odriozola-Gil Y., Lara E., Jimeno-González S., Suñé C., Muñoz-Centeno M.C., Reyes J.C, & Chávez S. (2021). Human prefoldin modulates co-transcriptional pre-mRNA splicing. Nucleic Acids Research, 49(11), 6267-6280.

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Antibody-based Immunoblotting Analysis

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Antibodies used for immunoblotting were anti-SRSF1 (1:1000, Invitrogen, 32–4500), anti-Rbfox1 (1:100, N-14, Santa Cruz Biotechnology, sc-135476), anti-Rbfox2 (1:2000, Bethyl Laboratories, A300-864A), anti-hnRNP F/H (1:500, Santa Cruz Biotechnology, sc-32310), anti-U1-70K (1:1000, Synaptic Systems, 203011), anti-U1A (1:1000, Thermo Fisher Scientific, PA5-27474), anti-U1C (1:1000, Sigma-Aldrich, SAB4200188), anti-GAPDH (1:2500, Sigma-Aldrich, G9545), anti-6xHis-tag (1:2500, Medical & Biological Laboratories, D291-3), anti-Flag M2 (1:1000, Sigma-Aldrich, F3165), anti-U2AF65 (1:400, MC3, Santa Cruz Biotechnology, sc-53942), anti-GFPT1 (1:500, Abcam, ab125069), anti-RL2 (1:800, Santa Cruz Biotechnology, sc-59624), anti-O-GlcNAc (CTD110.6) (1:2000, Cell Signaling Technology, 9875), anti-β-actin (1:1000, Santa Cruz Biotechnology, sc-47778), and anti-synaptophysin antibodies (1:50, Innovative Research, 18–0130). The secondary antibodies were anti-mouse IgG (1:2000, Cell Signaling Technology, 7076) conjugated to horseradish peroxidase, goat anti-rabbit IgG (1:2000, Cell Signaling Technology, 7074) conjugated to horseradish peroxidase, and Alexa 488-conjugated goat anti-rabbit antibody (1:1000, Thermo Fisher Scientific, A-11034). AChRs were visualized by Alexa 594-conjugated ɑ-bungarotoxin (1:1000, Invitrogen, B-13423).

Farshadyeganeh P., Nazim M., Zhang R., Ohkawara B., Nakajima K., Rahman M.A., Nasrin F., Ito M., Takeda J.I., Ohe K., Miyasaka Y., Ohno T., Masuda A, & Ohno K. (2023). Splicing regulation of GFPT1 muscle-specific isoform and its roles in glucose metabolisms and neuromuscular junction. iScience, 26(10), 107746.

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