Anti evi1

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-EVI1 is a primary antibody that recognizes the EVI1 protein, a transcriptional regulator involved in cellular processes. This antibody can be used to detect and quantify EVI1 expression in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-EVI1 antibody (2 citations)

4 protocols using anti evi1

EVI1 Chromatin Immunoprecipitation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chromatin immunoprecipitation was done with anti-EVI1 (#2593), IgG (#7076) and H3 (#9715) antibodies from Cell Signaling Technology (CST, Danvers, MA, USA) with the EZ-ChIP kit (#17–371) from Millipore (Etobicoke, ON, Canada) as directed. EVI1 binding enrichment at the +1379bp and -1000bp (negative control) sites was examined using quantitative PCR as previously described (Dzneladze et al., 2015). The following primers were used: EVI1 +1379bp 5’-TACCTTTGAACGGCTCCATC-3’ (sense) and 5’- CCCACTTCCTAGCCCCTAAC-3’ (antisense); EVI1 -1000bp 5’- TACTGGAAAACCCGGTAGG-3’ (sense) and 5’- CTGACAGGAAGGAGATATGCAA-3’ (antisense).

Dzneladze I., Woolley J.F., Rossell C., Han Y., Rashid A., Jain M., Reimand J., Minden M.D, & Salmena L. (2018). SubID, a non-median dichotomization tool for heterogeneous populations, reveals the pan-cancer significance of INPP4B and its regulation by EVI1 in AML. PLoS ONE, 13(2), e0191510.

+ Open protocol

+ Expand

Antibody Sources for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Specific antibodies were purchased from the following commercial sources: Anti-AFP, anti-ALB, anti-CD44, anti-Evi1, anti-flag (mouse), anti-HA, anti-HNF4α, anti-H3, anti-H3K36me3, anti-Myc, anti-OCT4A, anti-P300, anti-PPM1A (rabbit), anti-pSmad2 (S465/467), anti-pSmad2 (S245/250/255), anti-pSmad3 (s423/425), anti-Smad2, anti-Smad3 (rabbit), anti-SnoN, anti-Sox2, anti-TAT, and normal rabbit IgG from Cell Signaling Technology (Danvers, MA); anti-PPM1A (mouse), anti-SC35, and anti-SETD2 were from Abcam (Cambridge, MA); Anti-Smad4 and normal mouse IgG were from Santa Cruz Biotechnology (Santa Cruz, CA); Anti-β-actin, and anti-flag (rabbit) from Sigma-Aldrich (St. Louis, MO); Alexa594 goat anti-mouse IgG from Life Technology (Carlsbad, CA); Dylight488 goat anti-rabbit IgG from Vector Labs (Burlingame, CA).

Zhang J., Han C., Song K., Chen W., Ungerleider N., Yao L., Ma W, & Wu T. (2020). The long-noncoding RNA MALAT1 regulates TGF-β/Smad signaling through formation of a lncRNA-protein complex with Smads, SETD2 and PPM1A in hepatic cells. PLoS ONE, 15(1), e0228160.

+ Open protocol

+ Expand

ChIP-seq assay for EVI1 transcription factor

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Assays were prepared using 10⁷ cells of each cell line per condition. Chromatin was fragmented by sonication (Bioruptor, Diagenode, Denville, NJ, USA) for 30 min (30-s pulses, 30-s pauses) and assays were carried out following the manufacturer's protocol (kch-mahigh-A16, HighCell# ChIP Kit, Diagenode), using anti-EVI1 (#2593, Cell Signaling Technology) or an equal amount of IgG isotype as negative control (#2729, Cell Signaling Technology). The amount of DNA was analyzed by real-time polymerase chain reactions using SYBR Green-based assays (Applied Biosystems, Life Technologies, Foster City, CA, USA). The results were calculated using the ΔCt method. A genomic region of the GAPDH gene was used as negative control (Diagenode). The corresponding human genome coordinates, EVI1-binding sites and primers designed for the assays are detailed in Supplementary Table 5. Whole-genome ChIP data were obtained by hybridization to SurePrint G3 Human Promoter 1x1M microarrays (IRB Core Facility) and analyzed by MACS (version 2.0.9).^{60 (link)} The data have been deposited under the GEO reference GSE50905. The complete ranking of differential EVI1 binding between adapted and sensitive MCF7 or HCC1937 cells was used as input for the GSEA of transcription factor targets.

Mateo F., Arenas E.J., Aguilar H., Serra-Musach J., de Garibay G.R., Boni J., Maicas M., Du S., Iorio F., Herranz-Ors C., Islam A., Prado X., Llorente A., Petit A., Vidal A., Català I., Soler T., Venturas G., Rojo-Sebastian A., Serra H., Cuadras D., Blanco I., Lozano J., Canals F., Sieuwerts A.M., de Weerd V., Look M.P., Puertas S., García N., Perkins A.S., Bonifaci N., Skowron M., Gómez-Baldó L., Hernández V., Martínez-Aranda A., Martínez-Iniesta M., Serrat X., Cerón J., Brunet J., Barretina M.P., Gil M., Falo C., Fernández A., Morilla I., Pernas S., Plà M.J., Andreu X., Seguí M.A., Ballester R., Castellà E., Nellist M., Morales S., Valls J., Velasco A., Matias-Guiu X., Figueras A., Sánchez-Mut J.V., Sánchez-Céspedes M., Cordero A., Gómez-Miragaya J., Palomero L., Gómez A., Gajewski T.F., Cohen E.E., Jesiotr M., Bodnar L., Quintela-Fandino M., López-Bigas N., Valdés-Mas R., Puente X.S., Viñals F., Casanovas O., Graupera M., Hernández-Losa J., Ramón y Cajal S., García-Alonso L., Saez-Rodriguez J., Esteller M., Sierra A., Martín-Martín N., Matheu A., Carracedo A., González-Suárez E., Nanjundan M., Cortés J., Lázaro C., Odero M.D., Martens J.W., Moreno-Bueno G., Barcellos-Hoff M.H., Villanueva A., Gomis R.R, & Pujana M.A. (2016). Stem cell-like transcriptional reprogramming mediates metastatic resistance to mTOR inhibition. Oncogene, 36(19), 2737-2749.

+ Open protocol

+ Expand

Immunohistochemistry of Pancreatic Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pancreas tissue and cell pellets were fixed overnight at room temperature in 10% neutral-buffered formalin (KliniPath, Olen, Belgium). Next, samples were washed twice with PBS, dehydrated, and embedded in paraffin. Sections of 4 µm thickness were cut. Automated stainings were performed on the Leica Bond RX™. Primary antibodies used are: anti-EVI-1 (1/1000, C50E12, Cell Signaling Technology, Danvers, MA, USA), anti-cleaved caspase 3 (1/200, 9661, Cell Signaling Technology), anti-CD3 (1/50, A0452, Agilent), anti-Krt19 (1/100, TromaIII, Developmental Studies Hybridoma Bank, Iowa City, IA, USA), anti-F4/80 (1/100, APC clone BM8.1, Sigma-Aldrich), anti-CD142 (1/100, AF2419, Bio-techne, Minneapolis, MN, USA), anti-Krt19 (1/20, M0888, Agilent, Santa-Clara, CA, USA), anti-CD19 (1/500, ab245235, Abcam), anti-collagen IV (1/500, 1340-01, Southern Biotechnology Associates) Trichrome Masson’s staining was performed automated at the Pathology Department of UZ Brussel, Brussels, Belgium. DNA staining was performed with DAPI (Agilent) or Hoechst.

Backx E., Wauters E., Baldan J., Van Bulck M., Michiels E., Heremans Y., De Paep D.L., Kurokawa M., Goyama S., Bouwens L., Jacquemin P., Houbracken I, & Rooman I. (2021). MECOM permits pancreatic acinar cell dedifferentiation avoiding cell death under stress conditions. Cell Death and Differentiation, 28(9), 2601-2615.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!