Proteins were run in 10% SDS‐polyacrylamide gels and transferred to supported nitrocellulose membranes by standard techniques. Membranes were incubated with the mouse monoclonal anti‐VEGFR‐1 (clone D2, 1:500; Santa Cruz Biotechnology), rabbit polyclonal anti‐Erk1&2 (1:1000; Genetex), rabbit polyclonal anti‐phospho‐Erk1&2 (Thr/Tyr185/187, 1:1000; Invitrogen) or rabbit polyclonal anti‐β‐tubulin (1:10 000; Santa Cruz Biotechnology) as primary antibodies. Immunodetection was performed using antimouse or anti‐rabbit Ig/Horseradish peroxidase secondary antibodies and ECL Western blotting detection reagents from GE Healthcare (Milan, Italy).
Antimouse or anti rabbit ig horseradish peroxidase secondary antibodies
Manufactured by GE Healthcare
Sourced in Italy
Antimouse or anti-rabbit Ig/Horseradish peroxidase secondary antibodies are laboratory reagents designed to detect and visualize target proteins in various immunoassay techniques. These antibodies are conjugated with the enzyme horseradish peroxidase, which can catalyze a chromogenic reaction, allowing for the specific detection and quantification of the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
Ecl western blotting detection reagent, by GE Healthcare (2 mentions)
Mouse monoclonal anti vegfr 1 clone d2, by Santa Cruz Biotechnology (2 mentions)
Anti β tubulin, by Santa Cruz Biotechnology (1 mentions)
Rabbit polyclonal anti erk1 2, by GeneTex (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti erk1 2, by GeneTex (1 mentions)
2 protocols using antimouse or anti rabbit ig horseradish peroxidase secondary antibodies
1
Western Blot Analysis of VEGFR-1 and ERK
2
Western Blot Analysis of VEGFR-1 and EGFR
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Proteins were run in 10% SDS-polyacrylamide gels and transferred to supported nitrocellulose membranes by standard techniques. Immunodetection was performed using the following antibodies: mouse monoclonal anti-VEGFR-1 (clone D2, 1:500; Santa Cruz Biotechnology, Santa Cruz, CA); mouse monoclonal anti-EGF receptor (EGFR) (528, 1:1000; Santa Cruz Biotechnology); mouse monoclonal antibody anti-EGFRvIII (L8A4; 1:1000; Absolute Antibody, Oxford, UK); rabbit polyclonal anti-phosphorylated VEGFR-1 at tyrosine 1213 (1:500; R&D Systems); rabbit polyclonal anti-Erk1&2 (1:1000; Genetex, Irvine, CA); rabbit polyclonal anti-phospho-Erk1&2 (Thr/Tyr185/187, 1:1000; Invitrogen); or rabbit polyclonal anti-β-actin (1:10,000; Sigma Aldrich) primary antibodies. Anti-mouse or anti-rabbit Ig/Horseradish peroxidase secondary antibodies and ECL Western blotting detection reagents from GE Healthcare (Milan, Italy) were used to identify the proteins of interest.
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