Patient-derived GSCs and human neural progenitor cells (hNPC) were cultured in human neural stem cell maintenance media (Millipore), 1% penicillin-streptomycin (PS), and supplemented with EGF and β fibroblast growth factor (20 ng/ml each). hNPC was induced from human embryonic stem cells H1 as described previously [71 (link)]. Noncancerous mouse astrocytes (C8-D1A) were cultured with Dulbecco’s modified Eagle’s medium/nutrient mixture F12 (DMEM/F12) with 10% fetal bovine serum (FBS) and 1% PS. Human embryonic kidney (HEK) 293T cells were cultured with DMEM-high glucose with 10% FBS and 1% PS. The following inhibitors were used in this study: 17-AAG (MedChemExpress), Z-VAD-FMK (MedChemExpress), KN-93 (MedChemExpress), Volasertib (Selleckchem), BI 2536 (MedChemExpress), KU-55933 (MedChemExpress), and Nocodazole (Sigma).
Human neural stem cell maintenance media
Manufactured by Merck Group
The Human neural stem cell maintenance media is a specialized cell culture medium designed to support the growth and maintenance of human neural stem cells in vitro. It provides the necessary nutrients, growth factors, and other components to sustain the undifferentiated state of these cells.
Automatically generated - may contain errors
Lab products found in correlation
Ku 55933, by MedChemExpress (1 mentions)
17 aag, by MedChemExpress (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Volasertib, by Selleck Chemicals (1 mentions)
Bi2536, by MedChemExpress (1 mentions)
Z vad fmk, by MedChemExpress (1 mentions)
Lovastatin hydroxy acid, by Cayman Chemical (1 mentions)
Sulconazole nitrate, by Selleck Chemicals (1 mentions)
Cholesterol methyl β cyclodextrin, by Merck Group (1 mentions)
2 protocols using human neural stem cell maintenance media
1
Culturing Patient-derived GSCs and hNPCs
2
Patient-Derived Glioblastoma Stem Cell Culture
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Patient-derived GSCs were provided by C. Brennan (Memorial Sloan Kettering Cancer Center) and R. A. DePinho at MD Anderson Cancer Center. The GSCs were cultured in human neural stem cell maintenance media (Millipore), 1% penicillin-streptomycin (PS), and supplemented with EGF and β fibroblast growth factor (20 ng ml−1 each). Noncancerous mouse astrocytes (C8-D1A) were provided by T. V. Arumugam (La Trobe University) and cultured with Dulbecco’s modified Eagle’s medium/nutrient mixture F12 (DMEM/F12) with 10% fetal bovine serum (FBS) and 1% PS. Human embryonic kidney–293 (HEK-293) T cells were cultured with DMEM with 10% FBS and 1% PS. The following inhibitors were used in this study: sulconazole nitrate (Selleckchem), fluconazole and tebuconazole (Sigma-Aldrich), lovastatin hydroxy acid (Cayman), and DCA (Sigma-Aldrich). Cholesterol–methyl-β-cyclodextrin (Sigma-Aldrich) was used in rescue experiments.
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