The smear negative and discrepant samples were processed in MGIT 960 culture tube. A 500 µl sample was taken out from decontaminated sample and inoculated in BACTEC MGIT 960 tube. After the culture flashed positive, MGIT tubes were confirmed for acid fast bacilli by ZN staining and further subjected to confirm as M. tuberculosis complex using Capilia TB Neo (TAUNS Corporation, Japan) and checked for contamination by growth on blood agar medium for 48 h at 37 °C ([6] , [11] (link), [21] ). The confirmed positive MGIT tube was processed with the Genotype MTBDRplus V.2.0 assay (Hain Life Science, Nehren, Germany) as per the manufacturer's protocol.
Mgit 960 tube
Manufactured by BD
The MGIT 960 tube is a laboratory equipment used for the automated and continuous monitoring of bacterial growth in liquid culture media. It provides an efficient and standardized method for the detection and identification of mycobacteria, including the tuberculosis-causing bacteria.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mgit 960 tube
1
Mycobacterium tuberculosis Detection Protocol
2
Isolation and Characterization of MTB
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
From each Bactec MGIT-960 tube, 200 μL of culture suspension was sub-cultured on L-J medium slants and incubated at 37°C to obtain pure growth (isolated colonies) of MTB. After 21–28 days of incubation, single colony with the help of sterile inoculating loop was picked from L-J medium and inoculated in the MGIT (Mycobacteria growth indicator tube). The tube was incubated in the Bactec MGIT-960 system until flagged positive and this growth was used for second-line MGIT DST and DNA extraction for spoligotyping and MTBDRsl20 (link).
3
Mycobacterial Culture and Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Clinical specimens were decontaminated using NaOH/NALC method as recommended by the United States Center for Disease Control and Prevention [13] . The laboratory used commercially available media for all mycobacterial culture (Becton Dickenson, Franklin Lakes, NJ, USA). Each specimen was cultured on solid (Lowenstein-Jensen slant) and liquid (BACTEC MGIT 960 tube) media. Capilia TB-Neo (Tauns Laboratories Inc., Izunokuni, Japan) or SD-Bioline (Standard Diagnostics Inc., Yongin, Korea) rapid tests for detection of Ag MPT64 were used to identify MTB isolates.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!