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Taq rna polymerase

Manufactured by Merck Group

Taq RNA polymerase is an enzyme used in molecular biology for the amplification of RNA sequences through reverse transcription and PCR. It is derived from the thermophilic bacterium Thermus aquaticus and exhibits optimal activity at high temperatures.

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2 protocols using taq rna polymerase

1

Apoptosis and Cytokine Analysis

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Cell culture media were purchased from Life technologies Corporation (Carlsbad, CA). Fetal bovine sera and Taq RNA polymerase were from Sigma-Aldrich (St Louis, MO). Antibodies were from Cell Signaling Technology (Danvers, MA). DuraScribe T7 Transcription kits were from Epicentre (Madison, WI). Recombinant human CD44 and EpCAM proteins were from Thermo Fisher Scientific. Alexa Fluor 488 Annexin V/Dead Cell apoptosis kits were from Invitrogen. ELISA Kits for detection of IFNα and TNFα were obtained from RayBiotech (Norcross, GA). TUNEL assay kits were purchased from R&D systems (Minneapolis, MN). CD44 siRNA and EpCAM siRNA were purchased from Life Technologies Corporation.
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2

Molecular Profiling Techniques for HER2/HER3 Signaling

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Cell culture media were purchased from Life Technologies (Carlsbad, CA). Fetal bovine sera and Taq RNA polymerase were from Sigma-Aldrich (St. Louis, MO). Antibodies were from Cell Signaling Technology (Danvers, MA). Single-stranded DNAs were synthesized by IDT (Coralville, IA). Transcript Aid T7 High Yield Transcription Kits were ordered from Thermo Fisher Scientific. HER2 and HER3 siRNAs were purchased from Life Technologies. Alexa Fluor 488 Annexin V/Dead Cell apoptosis kits were from Invitrogen. ELISA kits for detection of IFN-α and IL-6 were obtained from RayBiotech (Norcross, GA). TUNEL assay kits were purchased from R&D systems (Minneapolis, MN). 2′-Fluoro-2′-deoxycytidine-5′-triphosphate, 2′-fluoro-2′-deoxyuridine-5′-triphosphate, and Cy5-labeled 2′-fluoro-labeled HER2 aptamer were purchased from TriLink Biotechnologies (San Diego, CA).
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