Luna 3u c18 2 100a

The firmness was measured using a texture analyser (TA.XT2i; Stable Micro Systems, https://www.stablemicrosystems.com) fitted with a 5‐mm flat probe. Each fruit was penetrated to 5 mm in depth at a speed rate of 0.5 mm sec⁻¹ and the maximum force developed during the test was recorded in Newton (N). Each fruit was measured twice at opposite sides of its equatorial zone. LC‐MS was performed using an Agilent 1100 HPLC/UV system (Agilent Technologies, https://www.agilent.com) with a reverse‐phase column (Luna 3u C18(2) 100A, 150 × 2 mm; Phenomenex, https://www.phenomenex.com) and connected to a Bruker esquire3000plus ion‐trap mass spectrometer (Bruker, https://www.bruker.com). LC‐MS analysis was performed according to the protocol described by Ring et al. (2013 (link)). The values were expressed as per mil (‰) equivalent internal standard per dry weight using biochanin as internal standard.

Levels of secondary metabolites were determined on an Agilent 1100 HPLC/UV system (Agilent Technologies, Germany) equipped with a reverse-phase column (Luna 3 u C18(2) 100 A, 150 × 2 mm; Phenomenex, Germany), a quaternary pump, and a variable wavelength detector. Connected to the HPLC was a Bruker esquire3000plus ion-trap mass spectrometer (Bruker Daltonics, Germany). HPLC and mass spectrometry were performed at optimized conditions33 (link)95 (link). Resulting data were analyzed with Data Analysis 5.1 software (Bruker Daltonics, Germany), and metabolites were identified using the in-house database. Levels (per mil equivalents of the dry weight, ‰ equ. dw.) of secondary metabolites quantified during targeted analyses are summarized in Supplementary Tables S4 and S5.