Chive seeds as well as seedlings before entering the greenhouse were sampled and analyzed. Twenty grams of seeds or seedling leaves were weighed into a stomacher bag containing 100 mL of either BPW or EE broth and homogenized in a Smasher (Biomérieux, Marcy l'Etoile, France) for 3 min. From the resulting BPW supernatant, appropriate volumes and dilutions as determined in pre-experiments were plated on R2A plates with and without antibiotics. Both BPW and EE homogenates were then incubated for enrichment and streaked onto antibiotic-containing selective media as described above.
Smasher
Manufactured by bioMérieux
Sourced in France
The Smasher is a laboratory equipment designed to homogenize solid and semi-solid samples. It utilizes a high-speed motor to drive a set of blades that thoroughly mix and disrupt the sample, preparing it for further analysis. The Smasher is a versatile tool suitable for a wide range of applications in clinical, food, and industrial laboratories.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using smasher
1
Chive Seed and Seedling Microbial Analysis
2
Microbiological Analysis of Food Samples
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For microbiological analysis, 10 g of sample were transferred into a bag blender Smasher® (bioMérieux, Marcy-l’Étoile, France) and 10-fold diluted and homogenized in physiological saline (0.85% NaCl and 0.1 % Bacto Peptone (Becton Dickinson, Sparks, MD, USA)) for 60 s with a SmasherTM device (bioMérieux Espãna S.A, Madrid, Spain). Serial decimal dilutions were prepared in physiological saline. Enumeration of S. aureus was performed on selective and differential chromogenic agar (CHROMagar Staphylococcus, CHROMagar, Paris, France) incubated at 37 °C for 48 h. LAB levels were determined in Man–Rogosa–Sharpe (MRS) agar (Merck, Darmstadt, Germany), incubated at 30 °C for 72 h anaerobically in sealed jars with an AnaeroGen sachet (Oxoid Ltd.)
The aw was measured with an AquaLabTM instrument (Series 3; Decagon Devices Inc., Pullman, WA, USA). The pH was measured with a penetration probe (52-32; Crison Instrument SA, Alella, Spain) connected to a portable pH meter (PH25; Crison Instruments). The detection of SEs was determined according to ISO 19020 [24 ] by automated immunofluorescence.
The gas concentration of MAP-packaged samples was measured with the gas analyzer PBI Dansensor CheckMate II (Ametek Instrumentos, S.L.U., Barcelona).
The aw was measured with an AquaLabTM instrument (Series 3; Decagon Devices Inc., Pullman, WA, USA). The pH was measured with a penetration probe (52-32; Crison Instrument SA, Alella, Spain) connected to a portable pH meter (PH25; Crison Instruments). The detection of SEs was determined according to ISO 19020 [24 ] by automated immunofluorescence.
The gas concentration of MAP-packaged samples was measured with the gas analyzer PBI Dansensor CheckMate II (Ametek Instrumentos, S.L.U., Barcelona).
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