For deep sequencing, 500 ng of the purified and pooled PCR genome amplicons were prepared for sequencing via Nextera DNA Flex Kit (Illumina, #20018705) with 96 indexes (Illumina, #20018708) according to the manufacturer’s protocol. Library quality was analyzed via Agilent 2200 TapeStation at the NCSU GSL. All sequencing was performed on an Illumina MiSeq instrument at North Carolina State University’s Genomic Science Laboratory to obtain paired end reads of approximately 300 base pairs.
96 indexes
Manufactured by Illumina
The 96 indexes are a set of short DNA sequences that can be used to uniquely identify different samples or libraries in a sequencing experiment. They are designed to work with Illumina's sequencing platforms and can be used to increase the throughput and flexibility of sequencing projects.
Automatically generated - may contain errors
2 protocols using 96 indexes
1
Nextera DNA Flex Sequencing Protocol
2
Nextera DNA Flex Library Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For deep sequencing, 500 ng of the purified and pooled PCR genome amplicons were prepared for sequencing via Nextera DNA Flex Kit (Illumina, # 20018705) with 96 indexes (Illumina, # 20018708) according to the manufacturer's protocol. Library quality was analyzed via Agilent 2200 TapeStation at the NCSU GSL. All sequencing was performed on an Illumina MiSeq instrument at North Carolina State University's Genomic Science Laboratory to obtain paired end reads of approximately 300 base pairs.
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