Serum IL‐17 concentration was measured for each sample in duplicate, using a commercially available IL‐17 ELISA kit for dogs (Canine IL‐17/IL‐17A Quantikine ELISA Kit, R&D Systems Inc, Minneapolis, Minnesota) and following the manufacturer's recommendations. The manufacturer previously had validated the kit using bilirubin‐spiking experiments, up to a serum bilirubin concentration of 1710 mmol/L without interference on the ELISA results (personal communication). No information was available with regard to potential interactions with hemoglobin in serum.
Samples were allotted randomly to the plates and the operator was blinded to the dog's group. Results were obtained using a commercially available spectrophotometer (Epoch Microplate Spectrophotometer, BioTek, Winooski, Vermont) and software interface (Gen5 Software, BioTek).
Samples were allotted randomly to the plates and the operator was blinded to the dog's group. Results were obtained using a commercially available spectrophotometer (Epoch Microplate Spectrophotometer, BioTek, Winooski, Vermont) and software interface (Gen5 Software, BioTek).