All solvents, starting materials, and reagents were acquired from commercial sources
(Sigma-Aldrich and Combi-Blocks). Robenidine was obtained from Santa Cruz Biotechnology
(Santa Cruz, California). All materials were used without further purification.
1H and 13C NMR spectra were taken on a Bruker 400 MHz instrument,
and chemical shifts are reported relative to TMS (0.0 ppm). Fluorescence measurements were
recorded using a Molecular Devices Spectramax iD3 equipped with Softmax Pro 7 software.
Final compounds were measured to be >95% pure by high performance liquid chromatography
(HPLC) using an Agilent Technologies 1260 Infinity II system (unless otherwise noted).
High-resolution mass spectrometry (HRMS) using electrospray ionization was performed by
the Portland State University BioAnalytical Mass Spectrometry Facility. Melting points
were measured using a Stanford Research Systems OptiMelt Automated Melting Point System
(model MPA100).
(Sigma-Aldrich and Combi-Blocks). Robenidine was obtained from Santa Cruz Biotechnology
(Santa Cruz, California). All materials were used without further purification.
1H and 13C NMR spectra were taken on a Bruker 400 MHz instrument,
and chemical shifts are reported relative to TMS (0.0 ppm). Fluorescence measurements were
recorded using a Molecular Devices Spectramax iD3 equipped with Softmax Pro 7 software.
Final compounds were measured to be >95% pure by high performance liquid chromatography
(HPLC) using an Agilent Technologies 1260 Infinity II system (unless otherwise noted).
High-resolution mass spectrometry (HRMS) using electrospray ionization was performed by
the Portland State University BioAnalytical Mass Spectrometry Facility. Melting points
were measured using a Stanford Research Systems OptiMelt Automated Melting Point System
(model MPA100).