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Gc grade hexane

Manufactured by Merck Group
Sourced in United States

GC-grade hexane is a high-purity solvent used in gas chromatography (GC) and other analytical applications. It is a clear, colorless liquid composed of linear alkane hydrocarbons. The product meets strict specifications for low levels of impurities to ensure reliable and consistent performance in GC analysis.

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3 protocols using gc grade hexane

1

Extraction and Characterization of Atlantic Salmon Oil

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Atlantic salmon (Salmo salar L.) was obtained from the local market (Timişoara, Romania) as a raw product in the spring of 2014. It was an aquaculture product of Norwegian origin. Only the meaty fish parts were used for oil extraction. GC-grade hexane (Sigma-Aldrich) was the main solvent used for raw and degraded fish oil dilutions. The Supelco 37 Component FAME mix (Sigma-Aldrich) and C8–C20 alkane standard solution (Fluka Chemie AG) were the main tools for identifying the FAMEs in derivatized raw and degraded fish oil. Anhydrous sodium sulfate (p.a., Merck & Co.) was used for drying the raw and degraded fish oil solutions. β-CD hydrate (>98%) from CycloLab (Budapest, Hungary) was used for ASO molecular encapsulation. Ethanol 96% (v/v, Chimopar, Bucharest, Romania) was used for β-CD complexation of ASO. A boron trifluoride–methanol complex (20%, Merck & Co., Inc.) was used for FA derivatization. Finally, Hydranal-Titrant 5, Hydranal-Solvent and Hydranal-Water Standard 10.0 (Sigma-Aldrich, Buchs, Switzerland) were used for the KFT water analysis of β-CD/ASO complexes.
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2

Quantitative Estimation of Foliage Terpene Content

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Terpene content was quantitatively estimated from untreated leaves. For the chemical analyses of foliage terpene content, six fresh leaf samples (120 ± 30 mg) were randomly chosen from individual plants and weighed into a 2 ml tube with 2.8 mm stainless steel beads (Bertin Technologies, Aix-en-Provence, France). Tubes with fresh samples were instantly frozen in liquid N2 and 1.5 ml GC-grade hexane (Sigma-Aldrich, St Louis, MO, USA) was added into each tube. The mixture was then immediately homogenized with a homogenizer (Precellys 24, Bertin Technologies) at 2400 g for 30 s at 25 °C. The homogenized plant material was incubated in a Thermo-Shaker (TS-100C, Biosan, Riga, Latvia) for 3 h at 1000 rpm at 25 °C. After incubation, samples were centrifuged (Universal 320R, Hettich, Tuttlingen, Germany) for 30 min at 13 200 g at 25 °C. An aliquot of 1 ml supernatant was pipetted into a 2 ml GC-MS glass vial (Sigma-Aldrich) for GC-MS analysis. A detailed description of GC-MS analysis and quantitative estimation of foliage terpene contents can be found in Kännaste et al. (2013) (link).
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3

Quantitative Analysis of Foliage Terpenes

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Terpene contents were quantitatively estimated from untreated leaves. For the chemical analyses of foliage terpene contents, six fresh leaf samples (120 ± 30 mg) were randomly chosen from individual plants and weighed into a 2 ml tube with 2.8 mm stainless-steel beads (Bertin Technologies, Aix-en-Provence, France). Tubes with fresh samples were instantly frozen in liquid N2 and then 1.5 ml GC-grade hexane (Sigma-Aldrich, St. Louis, MO, USA) was added into each tube. The mixture was then immediately homogenized with a homogenizer (Precellys 24, Bertin Technologies, Aix-en-Provence, France) at 6500 g for 30 seconds at 25°C. The homogenized plant material was incubated in a Thermo-Shaker (TS-100C, Biosan, Riga, Latvia) for 3 h at 1000 rpm at 25°C. After incubation, samples were centrifuged (Universal 320R, Hettich, Tuttlingen, Germany) for 30 min at 13000 g at 25°C. An aliquot of 1 ml supernatant was pipetted into a 2 ml GC-MS glass vial (Sigma-Aldrich, St. Louis, MO, USA) for GC-MS analysis. A detailed description of GC-MS analysis and quantitative estimation of foliage terpene contents can be found in Kännaste et al. (2013) (link).
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