The wood increment core of S. rubra Sr2 individual was cut by an electric saw into small wood blocks (~0.7 cm × 0.7 cm × 0.7 cm) corresponding to sapwood, transition zone and heartwood, respectively. Each block was then trimmed with a razor blade to generate a transverse cutting surface of approximately 1 mm × 2.5 mm which was left to be cut with an ultramicrotome (EM UC6, Leica Microsystèmes, SAS, Nanterre, France) using a diamond knife (DIATOME Cryotrim 45°, Leica Microsystèmes, SAS, Nanterre, France). A high cutting speed of 50 mm/s was used considering the dense transverse wood surface and the cutting feed was set at 200 nm. The clearance angle was kept constant at 6° during the sectioning. Optical images of the wood surfaces were acquired at 10×magnification with an Olympus BX51 microscope (Rungis, France) equipped with a motorized scanning stage (Marzhauser Wetzlar GmbH, Wetzlar, Germany) and a SC30 color camera, and monitored by the Olympus Stream Motion 1.9 software. Extended focal imaging (EFI) scanning mode was used to reveal the topography of the wood surface.
Sc30 color camera
Manufactured by Olympus
Sourced in Germany
The SC30 color camera is a digital camera designed for laboratory and research applications. It features a high-resolution sensor and can capture detailed images. The camera is capable of producing consistent and accurate color reproduction.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sc30 color camera
1
Transverse Wood Surface Imaging
2
Wood Surface Characterization Protocol
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Before analysis, the bark was removed and the remaining stem was trimmed with a razor blade to generate a small block of about 1 cm × 1 cm × 1 cm. A transverse surface of approximately 1 mm × 3 mm was obtained with ultramicrotome (EM UC6, Leica Microsystèmes, SAS, Nanterre, France) using a diamond knife (DIATOME Cryotrim 45°, Leica Microsystèmes, SAS, Nanterre, France). The cutting was performed at a speed of 2 mm/s with a fixed cutting feed of 200 nm. The clearance angle was kept constant at 6° during the sectioning. Optical images of wood surfaces were acquired at ×10 magnification with an Olympus BX51 microscope (Rungis, France) equipped with a motorized scanning stage (Marzhauser Wetzlar GmbH, Wetzlar, Germany), a SC30 color camera, and monitored by the Olympus Stream Motion 1.9 software. Extended focal imaging (EFI) scanning mode was applied to improve the image quality.
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