Anti eif4e

Manufactured by Fortis Life Sciences

Anti-eIF4E is a laboratory reagent that binds to the eIF4E protein, which is involved in the initiation of protein synthesis. It can be used in research applications to study the role of eIF4E in cellular processes.

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Lab products found in correlation

Anti 4e bp1, by Cell Signaling Technology (3 mentions) Anti eif4a1, by Cell Signaling Technology (3 mentions) Anti eif4g1, by Cell Signaling Technology (3 mentions) Anti eif3d, by Fortis Life Sciences (3 mentions) Anti rps19, by Fortis Life Sciences (3 mentions) Anti c jun, by Cell Signaling Technology (3 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Anti rplp0, by Fortis Life Sciences (2 mentions) Anti hsp90, by BD (2 mentions) Nitrocellulose membrane, by Cytiva (1 mentions) Anti vimentin, by Santa Cruz Biotechnology (1 mentions) Anti pabpc1, by Abcam (1 mentions) Anti α tubulin, by Santa Cruz Biotechnology (1 mentions) Anti calnexin, by Enzo Life Sciences (1 mentions) Anti pgc 1α, by Novus Biologicals (1 mentions) Anti nono, by Fortis Life Sciences (1 mentions) Anti mhc, by Abcam (1 mentions) Anti myoglobin, by Santa Cruz Biotechnology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions)

4 protocols using anti eif4e

Western Blot Analysis of Protein Targets

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Cell lysates were electrophoresed in 8%, 10%, or 12% polyacrylamide gels, and proteins were transferred to a nitrocellulose membrane (Amersham Biosciences) by electroblotting. Proteins were detected using the following antibodies: anti-PGC-1β (Bethyl Laboratories, A302-273A), anti-PGC-1α (Novus, NBP1-04676), anti-Flag (Sigma-Aldrich, A8592), anti-β-actin (Sigma-Aldrich, A2228), anti-CBP80 (Bethyl Laboratories, A301-793A or, alternatively, Choe et al. 2010 (link)), anti-CBP20 (Santa Cruz Biotechnology, sc-48793), anti-PABPC1 (Abcam, ab21060), anti-eIF4E (Bethyl Laboratories, A301-153A), anti-α-Tubulin (Santa Cruz Biotechnology, sc-58666), anti-P62 (BabCO, mAb414), anti-vimentin (Santa Cruz Biotechnology, sc-6260), anti-pSer2-RNAPII (Abcam, ab5095), anti-GAPDH (Cell Signaling Technology, 2118), anti-NONO (Bethyl Laboratories, A300-582A), anti-myoglobin (Santa Cruz Biotechnology, sc25607), anti-MHC (Abcam, ab91506), or anti-calnexin (Enzo, SPA-865F).

Cho H., Rambout X., Gleghorn M.L., Nguyen P.Q., Phipps C.R., Miyoshi K., Myers J.R., Kataoka N., Fasan R, & Maquat L.E. (2018). Transcriptional coactivator PGC-1α contains a novel CBP80-binding motif that orchestrates efficient target gene expression. Genes & Development, 32(7-8), 555-567.

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Western Blot Antibody Characterization

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Western blot analyses were performed using the following antibodies: anti-eIF3d (Bethyl A301-758A), anti-eIF4A1 (Cell Signaling 2490), anti-eIF4G1 (Cell Signaling 2858), anti-rpS19 (Bethyl A304-002A), anti-eIF4E (Bethyl A301-154A), anti-rpLP0 (Bethyl A302-882A), anti-HA epitope tag (Pierce 26183), anti-c-Jun (Cell Signaling 9165), anti-Hsp90 (BD Biosciences 610418), and anti-4EBP1 (Cell Signaling 9644).

Lee A.S., Kranzusch P.J., Doudna J.A, & Cate J.H. (2016). eIF3d is an mRNA cap-binding protein required for specialized translation initiation. Nature, 536(7614), 96-99.

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Western Blot Antibody Characterization

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Lee A.S., Kranzusch P.J., Doudna J.A, & Cate J.H. (2016). eIF3d is an mRNA cap-binding protein required for specialized translation initiation. Nature, 536(7614), 96-99.

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Immunoblot Antibody Dilutions for Protein Analysis

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Immunoblot studies were performed using the following antibodies at 1:1000 dilution: anti-DAP5 (BD Biosciences 610742); anti-eIF3d (Bethyl A301-758A); anti-eIF4A1 (Cell Signaling 2490); anti-eIF4G1 (Cell Signaling 2858); anti-rpS19 (Bethyl A304-002A); anti-eIF4E (Bethyl A301-154A); anti-HA epitope tag (Abcam 18181); anti-c-Jun (Cell Signaling 9165); anti-CDK12 (Cell Signaling 11973); anti-MMP1 (Abcam 137332); anti-GAPDH (Cell Signaling 2118S); anti-4E-BP1 (Cell Signaling 9644); anti-Lumin A (Abcam 226189); anti-AUF1 (EMD Millipore 07260); anti FXR1 (Cell Signaling 12295); anti FXR2 (Cell Signaling 7098); and anti-PABP (Cell Signaling 4992). The following antibodies were used at 1:500 dilution: anti-ETS1 (Abcam 26096); anti-OSMR (Abcam 210771); anti-EGFR (Cell Signaling 4257); anti-SERPINE2 (Abcam 75348); anti-ITGV (Abcam 124968); and anti-p53-BP1 (Abcam 21083).

de la Parra C., Ernlund A., Alard A., Ruggles K., Ueberheide B, & Schneider R.J. (2018). A widespread alternate form of cap-dependent mRNA translation initiation. Nature Communications, 9, 3068.

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