Ciprofloxacin hcl

E. coli strains were purchased from the American Type Culture Collection (ATCC25922) or Dharmacon (BW25113). Typically, strains cultured in liquid yeast peptone dextrose (YPD) media (1% yeast extract, 2% peptone, and 2% glucose) or YPD plates (2% agar) at 37°C. YPD was chosen as the media for our studies as we found that the ~MIC90 of antidepressants were lower compared with Luria Broth (LB) and Tryptic Soy Broth (TSB) without changing the MICs of antibiotics (Fig. S1A). All antidepressant and antibiotic solutions were prepared from powder stocks. All antidepressants were purchased from TCI: fluoxetine hydrochloride, sertraline hydrochloride, amitriptyline hydrochloride, nortriptyline hydrochloride, clomipramine hydrochloride. The following antibiotics were purchased from the indicated companies: ampicillin sodium (GoldBio), chloramphenicol (Sigma-Aldrich), kanamycin sulfate (IBI Scientific), rifampicin (GoldBio), and ciprofloxacin HCl (GoldBio). All were dissolved in appropriate solvent (water or ethanol) to make stocks that were then diluted into media to make agar plates or liquid cultures. Other chemicals included resazurin sodium salt (Alfa Aesar) and Hoechst 33342 (Ana Spec).